Literature DB >> 3744441

Isoelectric focusing and reverse immunoblotting of autoantibodies against high molecular weight antigens.

D I Stott, J McLearie.   

Abstract

A method for the clonal analysis of antibodies against high M.Wt. antigens is described. It involves isoelectric focusing, electrophoretic transfer of the focused antibodies to a nitrocellulose membrane and detection of the membrane-bound antibodies by overlay with radiolabelled antigen (reverse immunoblotting). The application of this technique to the clonal analysis of autoantibodies against thyroglobulin and DNA is described.

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Year:  1986        PMID： 3744441     DOI： 10.3109/08820138609094137

Source DB:  PubMed          Journal:  Immunol Invest        ISSN： 0882-0139            Impact factor:   3.657


  4 in total

1.  Homogeneous immunoglobulins in the sera of lung carcinoma patients receiving cytotoxic chemotherapy--detection with the use of isoelectric focusing and immunoblotting.

Authors:  H Haas; A Lange; M Schlaak
Journal:  Clin Exp Immunol       Date:  1987-12       Impact factor: 4.330

2.  Analysis of the spectrotypes of autoantibodies against thyroglobulin in two rat models of autoimmune thyroiditis.

Authors:  D I Stott; R Hassman; L Neilson; A M McGregor
Journal:  Clin Exp Immunol       Date:  1988-08       Impact factor: 4.330

3.  Naturally occurring antibodies to bovine and human retinal S antigen: a comparison between uveitis patients and healthy volunteers.

Authors:  J V Forrester; D I Stott; K M Hercus
Journal:  Br J Ophthalmol       Date:  1989-02       Impact factor: 4.638

Review 4.  Immunoblotting and dot blotting.

Authors:  D I Stott
Journal:  J Immunol Methods       Date:  1989-05-12       Impact factor: 2.303
  4 in total

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