Literature DB >> 3743668

Immunofractionation of DNA sequences associated with HMG-17 in chromatin.

S Druckmann, E Mendelson, D Landsman, M Bustin.   

Abstract

Antibodies specific for chromosomal protein HMG-17 were immobilized on CNBr-Sepharose and the resulting immunoaffinity column was used to purify chromatin segments enriched in HMG-17. The DNA was purified from both the nucleosomal fraction which was bound to the column and from the fraction which was not bound, and examined with DNA probes representing repetitive DNA, non-transcribed genes, transcribed genes and inducible genes. The results suggest that HMG-17 is preferentially associated with DNA sequences coding for genes, regardless of whether they are transcribed, and therefore support the notion that HMG-17 confers specific structural characteristics on selected regions in the genome.

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Year:  1986        PMID： 3743668     DOI： 10.1016/0014-4827(86)90493-3

Source DB:  PubMed          Journal:  Exp Cell Res        ISSN： 0014-4827            Impact factor:   3.905


  14 in total

1.  Recombinant human chromosomal proteins HMG-14 and HMG-17.

Authors:  M Bustin; P S Becerra; M P Crippa; D A Lehn; J M Pash; J Shiloach
Journal:  Nucleic Acids Res       Date:  1991-06-11       Impact factor: 16.971

2.  Genomic profiling of HMGN1 reveals an association with chromatin at regulatory regions.

Authors:  Suresh Cuddapah; Dustin E Schones; Kairong Cui; Tae-Young Roh; Artem Barski; Gang Wei; Mark Rochman; Michael Bustin; Keji Zhao
Journal:  Mol Cell Biol       Date:  2010-12-20       Impact factor: 4.272

3.  Blotting Index of Dissimilarity: use to study immunological relatedness of plant and animal High Mobility Group (HMG) chromosomal proteins.

Authors:  S Spiker; K M Everett
Journal:  Plant Mol Biol       Date:  1987-09       Impact factor: 4.076

Review 4.  Immunochemical approaches to the study of histone H1 and high mobility group chromatin proteins.

Authors:  J S Zlatanova
Journal:  Mol Cell Biochem       Date:  1990-01-18       Impact factor: 3.396

5.  Neither HMG-14a nor HMG-17 gene function is required for growth of chicken DT40 cells or maintenance of DNaseI-hypersensitive sites.

Authors:  Y Li; J R Strahler; J B Dodgson
Journal:  Nucleic Acids Res       Date:  1997-01-15       Impact factor: 16.971

6.  Cell cycle regulated synthesis of an abundant transcript for human chromosomal protein HMG-17.

Authors:  M Bustin; N Soares; D Landsman; T Srikantha; J M Collins
Journal:  Nucleic Acids Res       Date:  1987-04-24       Impact factor: 16.971

7.  The chicken HMG-17 gene is dispensable for cell growth in vitro.

Authors:  Y Li; J B Dodgson
Journal:  Mol Cell Biol       Date:  1995-10       Impact factor: 4.272

8.  Modular structure of chromosomal proteins HMG-14 and HMG-17: definition of a transcriptional enhancement domain distinct from the nucleosomal binding domain.

Authors:  L Trieschmann; Y V Postnikov; A Rickers; M Bustin
Journal:  Mol Cell Biol       Date:  1995-12       Impact factor: 4.272

9.  Distribution of high mobility group proteins 1/2, E and 14/17 and linker histones H1 and H5 on transcribed and non-transcribed regions of chicken erythrocyte chromatin.

Authors:  Y V Postnikov; V V Shick; A V Belyavsky; K R Khrapko; K L Brodolin; T A Nikolskaya; A D Mirzabekov
Journal:  Nucleic Acids Res       Date:  1991-02-25       Impact factor: 16.971

10.  Assessment of the transcriptional activation potential of the HMG chromosomal proteins.

Authors:  D Landsman; M Bustin
Journal:  Mol Cell Biol       Date:  1991-09       Impact factor: 4.272
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