Literature DB >> 3743034

A sensitive ELISA for the quantitation of human C5a in blood plasma using a monoclonal antibody.

M Schulze, O Götze.   

Abstract

An ELISA for the quantitation of the C5a anaphylatoxin of complement in human plasma is presented which is based on the use of both a monoclonal antibody and polyclonal antibodies to C5a. Its detection limit is 1 ng C5a/ml plasma. It detects no C5a in fresh EDTA-plasma. Complete removal of C5 by an optimized precipitation step prior to the assay procedure is an essential feature of the method.

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Year:  1986        PMID： 3743034     DOI： 10.1159/000467875

Source DB:  PubMed          Journal:  Complement        ISSN： 0253-5076


  3 in total

1.  Functionally active complement proteins C6 and C7 detected in C6- and C7-deficient individuals.

Authors:  R Würzner; A Orren; P Potter; B P Morgan; D Ponard; P Späth; M Brai; M Schulze; L Happe; O Götze
Journal:  Clin Exp Immunol       Date:  1991-03       Impact factor: 4.330

2.  Complement activation in patients with renal failure as detected through the quantitation of fragments of the complement proteins C3, C5, and factor B.

Authors:  M Oppermann; M Haubitz; E Quentin; O Götze
Journal:  Klin Wochenschr       Date:  1988-09-15

3.  Systemic Inflammation and Complement Activation Parameters Predict Clinical Outcome of Severe SARS-CoV-2 Infections.

Authors:  Silke Huber; Mariam Massri; Marco Grasse; Verena Fleischer; Sára Kellnerová; Verena Harpf; Ludwig Knabl; Ludwig Knabl; Tatjana Heiner; Moritz Kummann; Magdalena Neurauter; Günter Rambach; Cornelia Speth; Reinhard Würzner
Journal:  Viruses       Date:  2021-11-26       Impact factor: 5.048
  3 in total

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