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Literature DB >> 3742350

In vitro response of purified ovine peripheral blood lymphocytes to phytohemagglutinin-M.

T Stirtzinger, V E Valli, R M Jacobs, L Beiner, B Jansen.

Abstract

Peripheral blood was collected from three normal yearling castrated male lambs. Lymphocytes were isolated by Ficoll-Hypaque gradient centrifugation. Lymphocyte response to phytohemagglutinin was evaluated in an isotopic uptake stimulation test under varying culture conditions in order to standardize the assay. Results were analyzed as log10 counts per minute and stimulation indices. Culture conditions consisting of 2 microL (20 micrograms) phytohemagglutinin-M/culture, 1 X 10(6) cells/mL and a 72 hour incubation period were selected for use in further studies.

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Year: 1986 PMID： 3742350 PMCID： PMC1255170

Source DB: PubMed Journal: Can J Vet Res ISSN： 0830-9000 Impact factor: 1.310

11 in total

1. Isolation of lymphocytes, granulocytes and macrophages.

Authors: A Bøyum
Journal: Scand J Immunol Date: 1976-06 Impact factor: 3.487

2. In vitro stimulation of ovine lymphocytes by various mitogens.

Authors: C Burrells; P W Wells
Journal: Res Vet Sci Date: 1977-07 Impact factor: 2.534

3. In vitro stimulation of bovine peripheral blood lymphocytes: standardization and kinetics of the response.

Authors: C C Muscoplat; A W Chen; D W Johnson; I Alhaji
Journal: Am J Vet Res Date: 1974-12 Impact factor: 1.156

4. Characteristics of lymphocyte responses to phytomitogens: comparison of responses of lymphocytes from normal and lymphocytotic cows.

Authors: C C Muscoplat; I Alhaji; D W Johnson; K A Pomeroy; J M Olson; V L Larson; J B Stevens; D K Sorensen
Journal: Am J Vet Res Date: 1974-08 Impact factor: 1.156

5. The role of cell-mediated immunity in infectious diseases of cattle?

Authors: R D Schultz
Journal: Adv Exp Med Biol Date: 1981 Impact factor: 2.622

6. The lymphocyte stimulation test in veterinary immunology.

Authors: F Kristensen; B Kristensen; S Lazáry
Journal: Vet Immunol Immunopathol Date: 1982-01 Impact factor: 2.046

7. Inhibition of lymphocyte blastogenesis by sera from cows with lymphoma.

Authors: R M Jacobs; V E Valli; B N Wilkie
Journal: Am J Vet Res Date: 1980-03 Impact factor: 1.156

8. Characterization of lymphocyte subpopulations in sheep by rosette formation, adherence to nylon wool and mitogen responsiveness.

Authors: P M Outteridge; S T Licence; R M Binns
Journal: Vet Immunol Immunopathol Date: 1981-02 Impact factor: 2.046