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Literature DB >> 3741392

Purification of the constituent enzyme fractions of mycobacillin synthetase.

S K Ghosh, N K Mukhopadhyay, S Majumder, S K Bose.

Abstract

The final purification of the three-fraction enzyme complex mycobacillin synthetase was done by hydroxyapatite column chromatography and sucrose-density-gradient centrifugation; each of the fractions obtained migrates as a single component in SDS/polyacrylamide-gel electrophoresis and gel electrofocusing. The Mr of the enzyme fractions A, B and C by gel filtration is 260 000, 190 000 and 105 000, and that by SDS/polyacrylamide-gel electrophoresis is 252 000, 198 000 and 108 000 respectively. None of the enzyme fractions appears to possess subunit structure.

Entities: Chemical

Mesh：

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Year: 1986 PMID： 3741392 PMCID： PMC1146651 DOI： 10.1042/bj2350081

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

21 in total

9. A rapid method for the preparation of the three enzymes of bacitracin synthetase essentially free from other proteins.

Authors: I Roland; O Froyshov; S G Laland
Journal: FEBS Lett Date: 1977-12-01 Impact factor: 4.124

10. Gramicidin S-synthetase. A further characterization of phenylalanine racemase, the light enzyme of gramicidin s-synthetase.

Authors: J Vater; H Kleinkauf
Journal: Biochim Biophys Acta Date: 1976-05-13

1 in total

1. Role of ATP and enzyme-bound nascent peptides in the control of elongation for mycobacillin synthesis.

Authors: S K Ghosh; S Majumder; N K Mukhopadhyay; S K Bose
Journal: Biochem J Date: 1986-11-15 Impact factor: 3.857

1 in total

Purification of the constituent enzyme fractions of mycobacillin synthetase.

1. Amino acid sequence in mycobacillin.

2. Gramicidin S-synthetase. Electrophoretic characterization of the multienzyme.

3. Bacitracin biosynthesis by three complementary fractions from Bacillus licheniformis.

4. Purification of the polyenzymes responsible for tyrocidine synthesis and their dissociation into subunits.

5. Interrelation between activation and polymerization in gramicidin S biosynthesis.

6. A rapid method for the preparation of pure heavy enzyme of gramicidin S synthetase.

7. Gramicidin S-synthetase. Preparation of the multienzymic complex with a high specific activity.

8. Properties and localisation of mycobacillin-synthesising enzyme system in Bacillus subtilis B3.

9. A rapid method for the preparation of the three enzymes of bacitracin synthetase essentially free from other proteins.

10. Gramicidin S-synthetase. A further characterization of phenylalanine racemase, the light enzyme of gramicidin s-synthetase.

1. Role of ATP and enzyme-bound nascent peptides in the control of elongation for mycobacillin synthesis.