Literature DB >> 3741377

Streptomyces K15 DD-peptidase-catalysed reactions with suicide beta-lactam carbonyl donors.

M Leyh-Bouille, M Nguyen-Distèche, S Pirlot, A Veithen, C Bourguignon, J M Ghuysen.   

Abstract

The values of the kinetic parameters that govern the interactions between the Streptomyces K15 DD-peptidase and beta-lactam compounds were determined by measuring the inactivating effect that these compounds exert on the transpeptidase activity of the enzyme and, in the case of [14C]benzylpenicillin and [14C]cefoxitin, by measuring the amounts of acyl-enzyme formed during the reaction. K15 DD-peptidase binds benzylpenicillin or cefoxitin at a molar ratio of 1:1. Benzylpenicilloate is the major product released during breakdown of the acyl-enzyme formed with benzylpenicillin. Benzylpenicillin is not a better acylating agent than the amide Ac2-L-Lys-D-Ala-D-Ala and ester Ac2-L-Lys-D-Ala-D-lactatecarbonyl-donor substrates. beta-Lactam compounds possessing a methoxy group on the alpha-face of the molecule show high inactivating potency.

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Year:  1986        PMID： 3741377      PMCID： PMC1146665          DOI： 10.1042/bj2350177

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  Beta-lactamase (Enterobacter species).

Authors:  G W Ross
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

2.  Beta-lactamases (Actinomycetes species).

Authors:  K Johnson; C Duez; J M Frère; J M Ghuysen
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

3.  Maturation of the head of bacteriophage T4. I. DNA packaging events.

Authors:  U K Laemmli; M Favre
Journal:  J Mol Biol       Date:  1973-11-15       Impact factor: 5.469

4.  Interaction between beta-lactam antibiotics and exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61.

Authors:  J M Frère; M Leyh-Bouille; J M Ghuysen; H R Perkins
Journal:  Eur J Biochem       Date:  1974-12-16

5.  The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis.

Authors:  R A Laskey
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Competition of beta-lactam antibiotics for the penicillin-binding proteins of Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella aerogenes, Proteus rettgeri, and Escherichia coli: comparison with antibacterial activity and effects upon bacterial morphology.

Authors:  N A Curtis; D Orr; G W Ross; M G Boulton
Journal:  Antimicrob Agents Chemother       Date:  1979-09       Impact factor: 5.191

7.  The peptidoglycan crosslinking enzyme system in Streptomyces strains R61, K15 and rimosus.

Authors:  M Leyh-Bouille; J Dusart; M Nguyen-Distèche; J M Ghuysen; P E Reynolds; H R Perkins
Journal:  Eur J Biochem       Date:  1977-11-15

8.  Interaction between monobactams and Streptomyces R61 DD-carboxypeptidase.

Authors:  N H Georgopapadakou; S A Smith; C M Cimarusti
Journal:  Eur J Biochem       Date:  1982-06

9.  Penicillins and cephalosporins are active site-directed acylating agents: evidence in support of the substrate analogue hypothesis.

Authors:  D J Waxman; R R Yocum; J L Strominger
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1980-05-16       Impact factor: 6.237

10.  Isolation of the membrane-bound 26 000-Mr penicillin-binding protein of Streptomyces strain K15 in the form of a penicillin-sensitive D-alanyl-D-alanine-cleaving transpeptidase.

Authors:  M Nguyen-Distèche; M Leyh-Bouille; J M Ghuysen
Journal:  Biochem J       Date:  1982-10-01       Impact factor: 3.857
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  15 in total

1.  CENTA as a chromogenic substrate for studying beta-lactamases.

Authors:  C Bebrone; C Moali; F Mahy; S Rival; J D Docquier; G M Rossolini; J Fastrez; R F Pratt; J M Frère; M Galleni
Journal:  Antimicrob Agents Chemother       Date:  2001-06       Impact factor: 5.191

Review 2.  Biochemistry and comparative genomics of SxxK superfamily acyltransferases offer a clue to the mycobacterial paradox: presence of penicillin-susceptible target proteins versus lack of efficiency of penicillin as therapeutic agent.

Authors:  Colette Goffin; Jean-Marie Ghuysen
Journal:  Microbiol Mol Biol Rev       Date:  2002-12       Impact factor: 11.056

3.  Secretion by overexpression and purification of the water-soluble Streptomyces K15 DD-transpeptidase/penicillin-binding protein.

Authors:  P Palomeque-Messia; V Quittre; M Leyh-Bouille; M Nguyen-Distèche; C J Gershater; I K Dacey; J Dusart; J Van Beeumen; J M Ghuysen
Journal:  Biochem J       Date:  1992-11-15       Impact factor: 3.857

4.  The Enterococcus hirae R40 penicillin-binding protein 5 and the methicillin-resistant Staphylococcus aureus penicillin-binding protein 2' are similar.

Authors:  A el Kharroubi; P Jacques; G Piras; J Van Beeumen; J Coyette; J M Ghuysen
Journal:  Biochem J       Date:  1991-12-01       Impact factor: 3.857

5.  Biochemical characterization of the 49 kDa penicillin-binding protein of Mycobacterium smegmatis.

Authors:  T Mukherjee; D Basu; S Mahapatra; C Goffin; J van Beeumen; J Basu
Journal:  Biochem J       Date:  1996-11-15       Impact factor: 3.857

6.  Active-site and membrane topology of the DD-peptidase/penicillin-binding protein no. 6 of Enterococcus hirae (Streptococcus faecium) A.T.C.C. 9790.

Authors:  A el Kharroubi; G Piras; P Jacques; I Szabo; J Van Beeumen; J Coyette; J M Ghuysen
Journal:  Biochem J       Date:  1989-09-01       Impact factor: 3.857

7.  Effects of thiol reagents on Streptomyces K15 DD-peptidase-catalysed reactions.

Authors:  M Leyh-Bouille; M Nguyen-Distèche; C Bellefroid-Bourguignon; J M Ghuysen
Journal:  Biochem J       Date:  1987-02-01       Impact factor: 3.857

8.  Streptomyces K15 active-site serine DD-transpeptidase: specificity profile for peptide, thiol ester and ester carbonyl donors and pathways of the transfer reactions.

Authors:  J Grandchamps; M Nguyen-Distèche; C Damblon; J M Frère; J M Ghuysen
Journal:  Biochem J       Date:  1995-04-15       Impact factor: 3.857

9.  Interactions between active-site-serine beta-lactamases and compounds bearing a methoxy side chain on the alpha-face of the beta-lactam ring: kinetic and molecular modelling studies.

Authors:  A Matagne; J Lamotte-Brasseur; G Dive; J R Knox; J M Frère
Journal:  Biochem J       Date:  1993-08-01       Impact factor: 3.857

10.  Kinetic study of two novel enantiomeric tricyclic beta-lactams which efficiently inactivate class C beta-lactamases.

Authors:  M Vilar; M Galleni; T Solmajer; B Turk; J M Frère; A Matagne
Journal:  Antimicrob Agents Chemother       Date:  2001-08       Impact factor: 5.191
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