Literature DB >> 3740416

Electrophoresis of 35S-labeled proteoglycans on polyacrylamide-agarose composite gels and their visualization by fluorography.

S L Carney, M T Bayliss, J M Collier, H Muir.   

Abstract

We have developed techniques for the electrophoresis of 35S-labeled proteoglycans on polyacrylamide-agarose gel slabs and subsequent fixation, impregnation, and fluorography of such electrophoretograms. The procedure permits the examination of newly synthesized proteoglycan subspecies using a rapid technique, previously unavailable for these labeled molecules.

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Year:  1986        PMID： 3740416     DOI： 10.1016/0003-2697(86)90150-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  17 in total

1.  Mature bovine articular cartilage contains abundant aggrecan that is C-terminally truncated at Ala719-Ala720, a site which is readily cleaved by m-calpain.

Authors:  Hidefumi Oshita; John D Sandy; Kiichi Suzuki; Atsushi Akaike; Yun Bai; Tomohiro Sasaki; Katsuji Shimizu
Journal:  Biochem J       Date:  2004-08-15       Impact factor: 3.857

2.  Synthesis and extracellular assembly of proteoglycans in human articular cartilage.

Authors:  M T Bayliss
Journal:  Agents Actions       Date:  1988-02

3.  Effects of tiaprofenic acid and other NSAIDs on proteoglycan metabolism in articular cartilage explants.

Authors:  H Muir; S L Carney; L G Hall
Journal:  Drugs       Date:  1988       Impact factor: 9.546

4.  A monoclonal antibody which recognizes a glycosaminoglycan epitope in both dermatan sulfate and chondroitin sulfate proteoglycans of human skin.

Authors:  J M Sorrell; D A Carrino; M A Baber; D Asselineau; A I Caplan
Journal:  Histochem J       Date:  1999-08

5.  Differential modulation of degradative and repair responses of interleukin-1-treated chondrocytes by platelet-derived growth factor.

Authors:  A K Harvey; S T Stack; S Chandrasekhar
Journal:  Biochem J       Date:  1993-05-15       Impact factor: 3.857

Review 6.  Isolation and purification of proteoglycans.

Authors:  N S Fedarko
Journal:  Experientia       Date:  1993-05-15

7.  Development of a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments: detection of fragments in human synovial fluids.

Authors:  A J Fosang; K Last; P Gardiner; D C Jackson; L Brown
Journal:  Biochem J       Date:  1995-08-15       Impact factor: 3.857

8.  Fibroblast and neutrophil collagenases cleave at two sites in the cartilage aggrecan interglobular domain.

Authors:  A J Fosang; K Last; V Knäuper; P J Neame; G Murphy; T E Hardingham; H Tschesche; J A Hamilton
Journal:  Biochem J       Date:  1993-10-01       Impact factor: 3.857

9.  Neutrophil collagenase (MMP-8) cleaves at the aggrecanase site E373-A374 in the interglobular domain of cartilage aggrecan.

Authors:  A J Fosang; K Last; P J Neame; G Murphy; V Knäuper; H Tschesche; C E Hughes; B Caterson; T E Hardingham
Journal:  Biochem J       Date:  1994-12-01       Impact factor: 3.857

10.  Metalloproteinase digestion of cartilage proteoglycan. Pattern of cleavage by stromelysin and susceptibility to collagenase.

Authors:  C Hughes; G Murphy; T E Hardingham
Journal:  Biochem J       Date:  1991-11-01       Impact factor: 3.857
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