Literature DB >> 3740411

Purification of type E botulinum neurotoxin by high-performance ion exchange chromatography.

J J Schmidt, L S Siegel.   

Abstract

A purification procedure for type E botulinum neurotoxin has been developed, based solely on high-performance ion exchange chromatography. The method exploits the differential chromatographic behavior of the free neurotoxin versus the neurotoxin-protein 12S complex. The high purity of the product was demonstrated with sodium dodecyl sulfate-gel electrophoresis and amino acid sequencing. Beginning with dialyzed crude extract, at least 4 mg of pure neurotoxin could be obtained in two working days. The method has been adapted to user-prepared columns for processing large volumes of crude neurotoxin in one batch.

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Year:  1986        PMID： 3740411     DOI： 10.1016/0003-2697(86)90175-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

1.  Botulinum neurotoxin types A, B, and E: fragmentations by autoproteolysis and other mechanisms including by O-phenanthroline-dithiothreitol, and association of the dinucleotides NAD(+)/NADH with the heavy chain of the three neurotoxins.

Authors:  Bibhuti R Dasgupta; Babu S Antharavally; William Tepp; Mary L Evenson
Journal:  Protein J       Date:  2005-08       Impact factor: 2.371

2.  Sequence homology between tetanus and botulinum toxins detected by an antipeptide antibody.

Authors:  J L Halpern; L A Smith; K B Seamon; K A Groover; W H Habig
Journal:  Infect Immun       Date:  1989-01       Impact factor: 3.441

3.  Simplified purification method for Clostridium botulinum type E toxin.

Authors:  J A Giménez; H Sugiyama
Journal:  Appl Environ Microbiol       Date:  1987-12       Impact factor: 4.792

4.  Characterization of the antibody response to the receptor binding domain of botulinum neurotoxin serotypes A and E.

Authors:  Michael R Baldwin; William H Tepp; Christina L Pier; Marite Bradshaw; Mengfei Ho; Brenda A Wilson; Robert B Fritz; Eric A Johnson; Joseph T Barbieri
Journal:  Infect Immun       Date:  2005-10       Impact factor: 3.441

5.  Botulinum type F neurotoxin. Large-scale purification and characterization of its binding to rat cerebrocortical synaptosomes.

Authors:  J D Wadsworth; M Desai; H S Tranter; H J King; P Hambleton; J Melling; J O Dolly; C C Shone
Journal:  Biochem J       Date:  1990-05-15       Impact factor: 3.857

6.  SV2 mediates entry of tetanus neurotoxin into central neurons.

Authors:  Felix L Yeh; Min Dong; Jun Yao; William H Tepp; Guangyun Lin; Eric A Johnson; Edwin R Chapman
Journal:  PLoS Pathog       Date:  2010-11-24       Impact factor: 6.823

7.  Human immune response to botulinum pentavalent (ABCDE) toxoid determined by a neutralization test and by an enzyme-linked immunosorbent assay.

Authors:  L S Siegel
Journal:  J Clin Microbiol       Date:  1988-11       Impact factor: 5.948

8.  Botulinum neurotoxins B and E translocate at different rates and exhibit divergent responses to GT1b and low pH.

Authors:  Shihu Sun; William H Tepp; Eric A Johnson; Edwin R Chapman
Journal:  Biochemistry       Date:  2012-07-02       Impact factor: 3.162

9.  Synaptotagmins I and II mediate entry of botulinum neurotoxin B into cells.

Authors:  Min Dong; David A Richards; Michael C Goodnough; William H Tepp; Eric A Johnson; Edwin R Chapman
Journal:  J Cell Biol       Date:  2003-09-22       Impact factor: 10.539
  9 in total

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