An evaluation of two methods of substrate alkalinization for the identification of Bordetella avium and other similar organisms.

The effect of using two different techniques for the detection of substrate alkalinization by Bordetella avium and reference strains of Alcaligenes faecalis and B. bronchiseptica was evaluated. The techniques used were those described by Otto and Pickett and Hinz et al. Each test was in tubes sealed by either screw caps or cotton wool plugs. The alkalinization patterns obtained depended upon both the technique and the type of seal used.