| Literature DB >> 3736391 |
P V Choudary, J A Barranger, S Tsuji, J Mayor, M E LaMarca, C L Cepko, R C Mulligan, E I Ginns.
Abstract
A recombinant retrovirus carrying complementary DNA containing the complete coding sequence for human glucocerebrosidase was constructed and used to infect mouse cells and human fibroblasts from a type 2 Gaucher patient. The cDNA stably integrated in the genome of the recipient cell. Infected cells made glucocerebrosidase-cross-reacting material, and the protein was glycosylated and contained the epitope(s) missing in the type 2 mutant glucocerebrosidase. This study demonstrates the feasibility of efficiently transferring the gene encoding glucocerebrosidase to Gaucher cells using retrovirus as vector.Entities:
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Year: 1986 PMID： 3736391
Source DB: PubMed Journal: Mol Biol Med ISSN： 0735-1313