Literature DB >> 3736320

A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase.

D De la Hoz, B P Doctor, J S Ralston, R S Rush, A D Wolfe.   

Abstract

A simple procedure has been developed for the large scale purification of fetal bovine serum acetylcholinesterase (AChE) (EC 3.1.1.7). The procedure involves two steps: batch adsorption of the AChE from 250 L of serum onto a procainamide affinity Sepharose 4B gel; and analytical procainamide affinity chromatography of the step-1 product. Over 100 mg of AChE was purified in 10 days to apparent homogeneity with this procedure.

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Year:  1986        PMID： 3736320     DOI： 10.1016/0024-3205(86)90530-8

Source DB:  PubMed          Journal:  Life Sci        ISSN： 0024-3205            Impact factor:   5.037


  9 in total

1.  Characterization of butyrylcholinesterase in bovine serum.

Authors:  Alicia J Dafferner; Sofya Lushchekina; Patrick Masson; Gaoping Xiao; Lawrence M Schopfer; Oksana Lockridge
Journal:  Chem Biol Interact       Date:  2017-02-08       Impact factor: 5.192

2.  LARGE SCALE PURIFICATION OF BUTYRYLCHOLINESTERASE FROM HUMAN PLASMA SUITABLE FOR INJECTION INTO MONKEYS; A POTENTIAL NEW THERAPEUTIC FOR PROTECTION AGAINST COCAINE AND NERVE AGENT TOXICITY.

Authors:  Oksana Lockridge; Lawrence M Schopfer; Gail Winger; James H Woods
Journal:  J Med Chem Biol Radiol Def       Date:  2005-07-01

3.  Characterization of butyrylcholinesterase from porcine milk.

Authors:  Ashima Saxena; Tatyana Belinskaya; Lawrence M Schopfer; Oksana Lockridge
Journal:  Arch Biochem Biophys       Date:  2018-06-15       Impact factor: 4.013

4.  Polyproline tetramer organizing peptides in fetal bovine serum acetylcholinesterase.

Authors:  Kevser Biberoglu; Lawrence M Schopfer; Ashima Saxena; Ozden Tacal; Oksana Lockridge
Journal:  Biochim Biophys Acta       Date:  2013-01-23

5.  Identification of amino acid residues involved in the binding of Huperzine A to cholinesterases.

Authors:  A Saxena; N Qian; I M Kovach; A P Kozikowski; Y P Pang; D C Vellom; Z Radić; D Quinn; P Taylor; B P Doctor
Journal:  Protein Sci       Date:  1994-10       Impact factor: 6.725

6.  Polyproline-rich peptides associated with Torpedo californica acetylcholinesterase tetramers.

Authors:  Lilly Toker; Israel Silman; Tzviya Zeev-Ben-Mordehai; Joel L Sussman; Lawrence M Schopfer; Oksana Lockridge
Journal:  Chem Biol Interact       Date:  2020-02-20       Impact factor: 5.192

7.  Purification of recombinant human butyrylcholinesterase on Hupresin®.

Authors:  Oksana Lockridge; Emilie David; Lawrence M Schopfer; Patrick Masson; Xavier Brazzolotto; Florian Nachon
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2018-10-25       Impact factor: 3.205

8.  Tetramer organizing polyproline-rich peptides identified by mass spectrometry after release of the peptides from Hupresin-purified butyrylcholinesterase tetramers isolated from milk of domestic pig (Sus scrofa).

Authors:  Ashima Saxena; Tatyana Belinskaya; Lawrence M Schopfer; Oksana Lockridge
Journal:  Data Brief       Date:  2018-08-31

9.  A highly stable minimally processed plant-derived recombinant acetylcholinesterase for nerve agent detection in adverse conditions.

Authors:  Yvonne J Rosenberg; Jeremy Walker; Xiaoming Jiang; Scott Donahue; Jason Robosky; Markus Sack; Jonathan Lees; Lori Urban
Journal:  Sci Rep       Date:  2015-08-13       Impact factor: 4.379
  9 in total

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