Literature DB >> 3735149

Purification and partial characterization of the H immobilization antigens of Tetrahymena thermophila.

F P Doerder, M S Berkowitz.   

Abstract

The H immobilization antigens specified by the SerH locus of Tetrahymena thermophila have been purified by a procedure utilizing acid fractionation, ammonium sulfate precipitation, gel filtration, and ion exchange chromatography. Purified antigen migrates as a single band on SDS-PAGE and IEF. Molecular weights of the four allelic H antigens range from 44,000 to 52,000, and isoelectric points range from 4.1 to 4.5. No carbohydrate was detected.

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Year:  1986        PMID: 3735149     DOI: 10.1111/j.1550-7408.1986.tb05590.x

Source DB:  PubMed          Journal:  J Protozool        ISSN: 0022-3921


  13 in total

1.  Multiple effects of mutation on expression of alternative cell surface protein genes in Tetrahymena thermophila.

Authors:  D L Smith; F P Doerder
Journal:  Genetics       Date:  1992-01       Impact factor: 4.562

2.  A temperature-sensitive mutation of the temperature-regulated SerH3 i-antigen gene of Tetrahymena thermophila: implications for regulation of mutual exclusion.

Authors:  G L LaCrosse; F P Doerder
Journal:  Genetics       Date:  1994-10       Impact factor: 4.562

3.  High frequency intragenic recombination during macronuclear development in Tetrahymena thermophila restores the wild-type SerH1 gene.

Authors:  J C Deak; F P Doerder
Journal:  Genetics       Date:  1998-03       Impact factor: 4.562

4.  mRNA stability plays a major role in regulating the temperature-specific expression of a Tetrahymena thermophila surface protein.

Authors:  H D Love; A Allen-Nash; Q A Zhao; G A Bannon
Journal:  Mol Cell Biol       Date:  1988-01       Impact factor: 4.272

5.  Nucleo-cytoplasmic interaction during macronuclear differentiation in ciliate protists: genetic basis for cytoplasmic control of SerH expression during macronuclear development in Tetrahymena thermophila.

Authors:  F P Doerder; M S Berkowitz
Journal:  Genetics       Date:  1987-09       Impact factor: 4.562

6.  DNA rearrangements associated with the H3 surface antigen gene of Tetrahymena thermophila that occur during macronuclear development.

Authors:  M M Tondravi
Journal:  Curr Genet       Date:  1988-12       Impact factor: 3.886

7.  Antisense ribosomes: rRNA as a vehicle for antisense RNAs.

Authors:  R Sweeney; Q Fan; M C Yao
Journal:  Proc Natl Acad Sci U S A       Date:  1996-08-06       Impact factor: 11.205

8.  Structure and expression of two temperature-specific surface proteins in the ciliated protozoan Tetrahymena thermophila.

Authors:  G A Bannon; R Perkins-Dameron; A Allen-Nash
Journal:  Mol Cell Biol       Date:  1986-09       Impact factor: 4.272

9.  Temperature regulation of the Tetrahymena mimbres glycosylphosphatidylinositol-anchored protein lipid composition.

Authors:  Y G Ko; C Y Hung; G A Thompson
Journal:  Biochem J       Date:  1995-04-01       Impact factor: 3.857

10.  Reproducible and variable rearrangements of a Tetrahymena thermophila surface protein gene family occur during macronuclear development.

Authors:  J P Kile; H D Love; C A Hubach; G A Bannon
Journal:  Mol Cell Biol       Date:  1988-11       Impact factor: 4.272

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