Enolase isoenzymes in human gliomas.

Gamma-enolase (one of the three possible subunits of the dimeric enzyme enolase (EC 4.2.1.11)) has been reported as a marker for human neurons. Studies investigating the presence of gamma-enolase in human gliomas have given conflicting results, but a definite finding is important for further studies of the biology of these tumors and the possible use of gamma-enolase as a marker for tumors originating in nervous tissue or for neuronal damage. Using electrophoresis of tumor tissue extracts as well as immunohistochemistry the authors have demonstrated the presence of gamma-enolase in human gliomas. Analysis of the gamma-enolase content in the plasma of patients with brain neoplasms further revealed that, although this enzyme may be present in the tumor itself, its concentration in blood is not a reliable marker for a tumor of the human central nervous system.