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Literature DB >> 3733217

In vitro assay for curative activity in blood of mice infected with Trypanosoma musculi.

Abstract

An in vitro assay for curative antibody present in plasma of mice cured of Trypanosoma musculi is described. The assay involves the addition of plasma to a sample of infected blood, followed by hourly monitoring of the parasite count therein. Immune plasma effected a significant reduction in parasite number, whereas normal mouse plasma did not. Heat treatment of immune plasma for 30 min at 56 degrees C abolished its ability to effect any reduction. The assay is simple, rapid, and economical and correlates well with results of all in vivo studies performed to date.

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Year: 1986 PMID： 3733217 PMCID： PMC260864 DOI： 10.1128/iai.53.2.240-244.1986

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

11 in total

1. The immunological response of CBA mice to Trypanosoma musculi: elimination of the parasite from the blood.

Authors: G A Targett; P Viens
Journal: Int J Parasitol Date: 1975-04 Impact factor: 3.981

2. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.

Authors: P L Ey; S J Prowse; C R Jenkin
Journal: Immunochemistry Date: 1978-07

3. The immunological response of CBA mice to Trypanosoma musculi. I. Initial control of the infection and the effect of T-cell deprivation.

Authors: P Viens; G A Targett; E Leuchars; A J Davies
Journal: Clin Exp Immunol Date: 1974-02 Impact factor: 4.330

In vitro assay for curative activity in blood of mice infected with Trypanosoma musculi.

1. The immunological response of CBA mice to Trypanosoma musculi: elimination of the parasite from the blood.

2. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.

3. The immunological response of CBA mice to Trypanosoma musculi. I. Initial control of the infection and the effect of T-cell deprivation.

4. Depletion of plasma complement in vivo by a protein of cobra venom: its effect on various immunologic reactions.

5. Characterization of antibodies mediating protection and cure of Trypanosoma musculi infection in mice.

6. Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.

7. Chronic infection with Trypanosoma musculi in congenitally athymic nude mice.

8. Cure of Trypanosoma musculi infection by heat-labile activity in immune plasma.

9. Trypanosoma musculi infection in B-cell-deficient mice.

10. The four subclasses of IgG can be isolated from mouse serum by using Protein A-Sepharose.

1. Further characterization of the curative antibodies in Trypanosoma musculi infection.