Morphological, histochemical and biochemical observations on the connective tissue matrix of in situ and isolated hepatic granulomas in experimental murine schistosomiasis.

The connective tissue matrix of hepatic granulomas from Swiss mice, each infected with 20 Schistosoma mansoni cercariae, was examined using morphological, histochemical and biochemical methods, during the evolution of the disease. Evidence was obtained by both light and electron microscopy that suggested the presence of two different collagen populations which appeared successively during granuloma evolution. Morphological features suggesting the occurrence of collagenolysis were observed at the final stage of granuloma development. The connective tissue matrix of schistosome granulomas was devoid of elastic system fibres. Schistosome egg granulomas contain dermatan sulphate-proteoglycan.