Literature DB >> 3728988

A sensitive spectrophotometric assay of pyruvate dehydrogenase activity.

P W Scislowski, E J Davis.   

Abstract

The recently reported highly sensitive method for assay of acetyl-CoA:arylamine N-acetyltransferase (EC 2.3.1.5) [H. H. Andres, A. J. Klein, S. M. Szabo, and W. W. Weber (1985) 145, 367-375] has been adapted for determination of pyruvate dehydrogenase activity. This method provides an improvement in sensitivity over extant spectrophotometric methods and circumvents limitations of assays using radioactive pyruvate. In addition, the assay is simple and inexpensive and can be readily adapted for measurement of enzyme activity in crude tissue extracts or homogenates.

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Year:  1986        PMID： 3728988     DOI： 10.1016/0003-2697(86)90454-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  2 in total

1.  Endogenous production of lipoic acid is essential for mouse development.

Authors:  Xianwen Yi; Nobuyo Maeda
Journal:  Mol Cell Biol       Date:  2005-09       Impact factor: 4.272

2.  Mitochondrial Liver Toxicity of Valproic Acid and Its Acid Derivatives Is Related to Inhibition of α-Lipoamide Dehydrogenase.

Authors:  Alexei P Kudin; Hafiz Mawasi; Arik Eisenkraft; Christian E Elger; Meir Bialer; Wolfram S Kunz
Journal:  Int J Mol Sci       Date:  2017-09-06       Impact factor: 5.923
  2 in total

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