Literature DB >> 3728985

Isolation and analysis of bacterial cobamides by high-performance liquid chromatography.

E Stupperich, I Steiner, M Rühlemann.   

Abstract

Cyanocobamides were extracted from diverse bacterial species, purified by XAD-4 and neutral aluminum oxide column chromatography, and separated by isocratic reversed-phase high-performance liquid chromatography (HPLC). Retention times are given for seven cobamide types: dicyanocobinamide (factor B), Co alpha-(alpha-benzimidazolyl)-Co beta-cyanocobamide, Co alpha-(5-hydroxybenzimidazolyl)-Co beta-cyanocobamide (factor III), Co alpha-(5-methoxybenzimidazolyl)-Co beta-cyanocobamide (factor IIIm), Co alpha-(5-methylbenzimidazolyl)-Co beta-cyanocobamide, cyanocob(III)alamin (vitamin B-12) and Co alpha-(naphthimidazolyl)-Co beta-cyanocobamide. Other Co beta-ligandyl-cobamides such as hydroxycobamide and the light-sensitive methyl-, acetyl-, propyl-, and adenosylcobamides were separated by HPLC in a gradient mode. The recovery of total cell cobamide after extraction, purification, and separation was 75-80%. The method was useful in preparative and analytical work. Less than 10 ng cyanocobamide was detectable.

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Year:  1986        PMID： 3728985     DOI： 10.1016/0003-2697(86)90447-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  12 in total

1.  N5-methyl-tetrahydromethanopterin:coenzyme M methyltransferase of Methanosarcina strain Gö1 is an Na(+)-translocating membrane protein.

Authors:  B Becher; V Müller; G Gottschalk
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490

2.  Fluorinated vitamin b(12) analogs are cofactors of corrinoid-dependent enzymes: a f-labeled nuclear magnetic resonance probe for identifying corrinoid-protein interactions.

Authors:  E Stupperich; H J Eisinger; R Kerssebaum; E Nexø
Journal:  Appl Environ Microbiol       Date:  1993-02       Impact factor: 4.792

3.  Evidence for the involvement of corrinoids and factor F430 in the reductive dechlorination of 1,2-dichloroethane by Methanosarcina barkeri.

Authors:  C Holliger; G Schraa; E Stupperich; A J Stams; A J Zehnder
Journal:  J Bacteriol       Date:  1992-07       Impact factor: 3.490

4.  Resolution of component proteins in an enzyme complex from Methanosarcina thermophila catalyzing the synthesis or cleavage of acetyl-CoA.

Authors:  D R Abbanat; J G Ferry
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

5.  Substitution of Co alpha-(5-hydroxybenzimidazolyl)cobamide (factor III) by vitamin B12 in Methanobacterium thermoautotrophicum.

Authors:  E Stupperich; I Steiner; H J Eisinger
Journal:  J Bacteriol       Date:  1987-07       Impact factor: 3.490

6.  Guided cobalamin biosynthesis supports Dehalococcoides mccartyi reductive dechlorination activity.

Authors:  Jun Yan; Jeongdae Im; Yi Yang; Frank E Löffler
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2013-03-11       Impact factor: 6.237

7.  Impact of vitamin B12 on formation of the tetrachloroethene reductive dehalogenase in Desulfitobacterium hafniense strain Y51.

Authors:  Anika Reinhold; Martin Westermann; Jana Seifert; Martin von Bergen; Torsten Schubert; Gabriele Diekert
Journal:  Appl Environ Microbiol       Date:  2012-09-07       Impact factor: 4.792

8.  Cobalamin-mediated mercury methylation by Desulfovibrio desulfuricans LS.

Authors:  S C Choi; R Bartha
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792

9.  Degradation and Fate of Carbon Tetrachloride in Unadapted Methanogenic Granular Sludge.

Authors: 
Journal:  Appl Environ Microbiol       Date:  1998-07-01       Impact factor: 4.792

10.  Vitamin B(12) and alpha-Ribonucleosides.

Authors:  Tilak Chandra; Kenneth L Brown
Journal:  Tetrahedron       Date:  2008-01-01       Impact factor: 2.457
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