Literature DB >> 3728983

Large-scale procedure for the purification of fibronectin domains.

L Borsi, P Castellani, E Balza, A Siri, C Pellecchia, F De Scalzi, L Zardi.   

Abstract

Human plasma fibronectin is composed of at least seven distinct domains, with affinities for different macromolecules and cell surfaces. Here we describe in detail a simple high-yield procedure for the purification of large amounts of fibronectin domains. This involves thermolysin digestion of the fibronectin molecule followed by the purification of the domain using mainly hydroxyapatite chromatography columns. This procedure represents a great simplification over those previously reported.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3728983     DOI： 10.1016/0003-2697(86)90443-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  14 in total

1.  Interaction of heparin with fibronectin and isolated fibronectin domains.

Authors:  K C Ingham; S A Brew; D H Atha
Journal:  Biochem J       Date:  1990-12-15       Impact factor: 3.857

2.  Interaction of the fibronectin COOH-terminal Fib-2 regions with fibrin: further characterization and localization of the Fib-2-binding sites.

Authors:  Evgeny Makogonenko; Kenneth C Ingham; Leonid Medved
Journal:  Biochemistry       Date:  2007-04-11       Impact factor: 3.162

3.  Affinity of human erythrocyte transglutaminase for a 42-kDa gelatin-binding fragment of human plasma fibronectin.

Authors:  J T Radek; J M Jeong; S N Murthy; K C Ingham; L Lorand
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-15       Impact factor: 11.205

4.  Binding of collagens to an enterotoxigenic strain of Escherichia coli.

Authors:  L Visai; P Speziale; S Bozzini
Journal:  Infect Immun       Date:  1990-02       Impact factor: 3.441

Review 5.  Dynamic structure of plasma fibronectin.

Authors:  Lisa M Maurer; Wenjiang Ma; Deane F Mosher
Journal:  Crit Rev Biochem Mol Biol       Date:  2016-05-17       Impact factor: 8.250

6.  A two-domain mechanism for group A streptococcal adherence through protein F to the extracellular matrix.

Authors:  V Ozeri; A Tovi; I Burstein; S Natanson-Yaron; M G Caparon; K M Yamada; S K Akiyama; I Vlodavsky; E Hanski
Journal:  EMBO J       Date:  1996-03-01       Impact factor: 11.598

7.  Transformed human cells produce a new fibronectin isoform by preferential alternative splicing of a previously unobserved exon.

Authors:  L Zardi; B Carnemolla; A Siri; T E Petersen; G Paolella; G Sebastio; F E Baralle
Journal:  EMBO J       Date:  1987-08       Impact factor: 11.598

8.  Lipoprotein(a) binds to fibronectin and has serine proteinase activity capable of cleaving it.

Authors:  E M Salonen; M Jauhiainen; L Zardi; A Vaheri; C Ehnholm
Journal:  EMBO J       Date:  1989-12-20       Impact factor: 11.598

9.  Modulation of matrix adhesive responses of human neuroblastoma cells by neighboring sequences in the fibronectins.

Authors:  G Mugnai; K Lewandowska; B Carnemolla; L Zardi; L A Culp
Journal:  J Cell Biol       Date:  1988-03       Impact factor: 10.539

10.  A tumor-associated fibronectin isoform generated by alternative splicing of messenger RNA precursors.

Authors:  B Carnemolla; E Balza; A Siri; L Zardi; M R Nicotra; A Bigotti; P G Natali
Journal:  J Cell Biol       Date:  1989-03       Impact factor: 10.539
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.