Literature DB >> 3727364

An improved hemagglutination test for study of canine parvovirus.

M Senda, N Hirayama, H Yamamoto, K Kurata.   

Abstract

Optimal conditions for hemagglutination (HA) by canine parvovirus (CPV) strains were investigated using several buffers. Porcine erythrocytes often agglutinated spontaneously in phosphate-buffered salt solution, isotonic saline solution or barbitone-complement-fixation buffer. Results were reproducible when borate-buffered saline (BBS) was used as the diluent for antigen, and "virus adjusting diluent" (VAD), containing 0.15 M NaCl and 0.3 M phosphate was used as the diluent for erythrocytes. Highest HA titers were obtained at pH 6.0 using BBS and VAD. Specific HA with CPV was observed not only at 4 degrees C but at 37 degrees C, and erythrocytes from horse, shrew mouse, hamster, cat, sheep and dog, as well as pig and African green monkey were agglutinated by CPV using the improved method.

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Year:  1986        PMID： 3727364     DOI： 10.1016/0378-1135(86)90035-0

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN： 0378-1135            Impact factor:   3.293


  8 in total

1.  Construction of an infectious DNA clone of the Y1 strain of canine parvovirus and characterization of the virus derived from the clone.

Authors:  M Horiuchi; M Shinagawa
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

2.  Isolation of canine parvovirus from a cat manifesting clinical signs of feline panleukopenia.

Authors:  M Mochizuki; M Horiuchi; H Hiragi; M C San Gabriel; N Yasuda; T Uno
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

3.  Vaccination against Feline Panleukopenia: implications from a field study in kittens.

Authors:  Verena Jakel; Klaus Cussler; Kay M Hanschmann; Uwe Truyen; Matthias König; Elisabeth Kamphuis; Karin Duchow
Journal:  BMC Vet Res       Date:  2012-05-21       Impact factor: 2.741

4.  Production and purification of VP2 protein of porcine parvovirus expressed in an insect-baculovirus cell system.

Authors:  Hongchao Zhou; Guizhe Yao; Shangjin Cui
Journal:  Virol J       Date:  2010-12-10       Impact factor: 4.099

5.  Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats.

Authors:  Andrea Balboni; Francesca Bassi; Stefano De Arcangeli; Rosanna Zobba; Carla Dedola; Alberto Alberti; Mara Battilani
Journal:  BMC Vet Res       Date:  2018-02-05       Impact factor: 2.741

6.  Discrepancy Between In-clinic and Haemagglutination-Inhibition Tests in Detecting Maternally-Derived Antibodies Against Canine Parvovirus in Puppies.

Authors:  Paola Dall'Ara; Stefania Lauzi; Joel Filipe; Roberta Caseri; Michela Beccaglia; Costantina Desario; Alessandra Cavalli; Giulio Guido Aiudi; Canio Buonavoglia; Nicola Decaro
Journal:  Front Vet Sci       Date:  2021-03-01

7.  Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies.

Authors:  R Puentes; N Eliopulos; R Pérez; G Franco; K Sosa; P Bianchi; A Furtado; S O Hübner; P A Esteves
Journal:  Braz J Microbiol       Date:  2012-06-01       Impact factor: 2.476

8.  Prevalence and risk factors for viral exposure in rural dogs around protected areas of the Atlantic forest.

Authors:  Nelson Henrique de Almeida Curi; Rodrigo Lima Massara; Ana Maria de Oliveira Paschoal; Amanda Soriano-Araújo; Zélia Inês Portela Lobato; Guilherme Ramos Demétrio; Adriano Garcia Chiarello; Marcelo Passamani
Journal:  BMC Vet Res       Date:  2016-01-28       Impact factor: 2.741
  8 in total

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