[3H]cyclohexyladenosine binding in rat brain: a pharmacological analysis using quantitative autoradiography.

Quantitative autoradiography was used to characterize the regional binding parameters of [3H]cyclohexyladenosine (CHA) and the regional inhibition of specific [3H]CHA binding observed with a series of adenosine agonists and antagonists. Under the conditions described [3H]CHA binding was best fit by a single, high affinity site model in all areas examined. No major regional differences were observed in the inhibition of [3H]CHA binding by the adenosine and xanthine analogs examined. Overall, the rank order of activity throughout all regions was CPA greater than R-PIA greater than NECA much greater than PACPX greater than or equal to 2-CADO greater than or equal to S-PIA much greater than DPX.