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Literature DB >> 3722127

Pectate lyase gene regulatory mutants of Erwinia chrysanthemi.

Abstract

The pelB gene, which encodes one of the five pectate lyase isoenzymes of Erwinia chrysanthemi 3937, was mutagenized with a mini-Mu transposable element that can form gene fusions to the neomycin phosphotransferase-encoding region. Secondary mutants resistant to kanamycin in the absence of polygalacturonate, an inducer of wild-type pectate lyase activities, were selected. Such mutants produced other pectate lyase isoenzymes in the absence of the inducer.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1986 PMID： 3722127 PMCID： PMC212893 DOI： 10.1128/jb.167.1.400-403.1986

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

13 in total

1. Synthesis and excretion of polygalacturonic acid trans-eliminase in Erwinia, Yersinia, and Klebsiella species.

Authors: A K Chatterjee; G E Buchanan; M K Behrens; M P Starr
Journal: Can J Microbiol Date: 1979-01 Impact factor: 2.419

Review 2. The genus Erwinia: enterobacteria pathogenic to plants and animals.

Authors: M P Starr; A K Chatterjee
Journal: Annu Rev Microbiol Date: 1972 Impact factor: 15.500

3. Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase.

Authors: T Andro; J P Chambost; A Kotoujansky; J Cattaneo; Y Bertheau; F Barras; F Van Gijsegem; A Coleno
Journal: J Bacteriol Date: 1984-12 Impact factor: 3.490

4. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors: J Vieira; J Messing
Journal: Gene Date: 1982-10 Impact factor: 3.688

5. Detection of depolymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves.

Authors: Y Bertheau; E Madgidi-Hervan; A Kotoujansky; C Nguyen-The; T Andro; A Coleno
Journal: Anal Biochem Date: 1984-06 Impact factor: 3.365

6. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors: C Yanisch-Perron; J Vieira; J Messing
Journal: Gene Date: 1985 Impact factor: 3.688

7. Construction and uses of a new transposable element whose insertion is able to produce gene fusions with the neomycin-phosphotransferase-coding region of Tn903.

Authors: P Ratet; F Richaud
Journal: Gene Date: 1986 Impact factor: 3.688

Pectate lyase gene regulatory mutants of Erwinia chrysanthemi.

1. Synthesis and excretion of polygalacturonic acid trans-eliminase in Erwinia, Yersinia, and Klebsiella species.

Review 2. The genus Erwinia: enterobacteria pathogenic to plants and animals.

3. Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase.

4. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

5. Detection of depolymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves.

6. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

7. Construction and uses of a new transposable element whose insertion is able to produce gene fusions with the neomycin-phosphotransferase-coding region of Tn903.

8. Lactose metabolism in Erwinia chrysanthemi.

9. Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes.

10. In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi.

1. Environmental conditions affect transcription of the pectinase genes of Erwinia chrysanthemi 3937.