Literature DB >> 372

Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures.

E Cabib, A Duran.   

Abstract

After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.

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Year:  1975        PMID： 372      PMCID： PMC236077          DOI： 10.1128/jb.124.3.1604-1606.1975

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  4 in total

1.  GENETIC ANALYSIS OF THE PHOSPHATASES IN ASPERGILLUS NIDULANS.

Authors:  G DORN
Journal:  Genet Res       Date:  1965-02       Impact factor: 1.588

2.  The acid phosphatase of yeast. Localization and secretion by protoplasts.

Authors:  W L MCLELLAN; J O LAMPEN
Journal:  Biochim Biophys Acta       Date:  1963-02-12

3.  High resolution colony staining for the detection of bacterial growth requirement mutants using naphthol azo-dye techniques.

Authors:  W Messer; W Vielmetter
Journal:  Biochem Biophys Res Commun       Date:  1965-10-26       Impact factor: 3.575

4.  Changes in the phosphatase activity of Baker's yeast during the growth phase and location of the phosphatases in the yeast cell.

Authors:  H SUOMALAINEN; M LINKO; E OURA
Journal:  Biochim Biophys Acta       Date:  1960-01-29
  4 in total
  18 in total

1.  A highly thermosensitive and permeable mutant of the marine yeast Cryptococcus aureus G7a potentially useful for single-cell protein production and its nutritive components.

Authors:  Tong Zhang; Zhenming Chi; Jun Sheng
Journal:  Mar Biotechnol (NY)       Date:  2008-09-19       Impact factor: 3.619

2.  Timing and function of chitin synthesis in yeast.

Authors:  E Cabib; B Bowers
Journal:  J Bacteriol       Date:  1975-12       Impact factor: 3.490

3.  A cdc-like autolytic Saccharomyces cerevisiae mutant altered in budding site selection is complemented by SPO12, a sporulation gene.

Authors:  G Molero; M Yuste-Rojas; A Montesi; A Vázquez; C Nombela; M Sanchez
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

4.  Genetic analysis of an osmotic sensitive Saccharomyces cerevisiae mutant.

Authors:  T Kozhina; L Stateva; P Venkov
Journal:  Mol Gen Genet       Date:  1979-03-05

5.  Septum formation, cell division, and sporulation in mutants of yeast deficient in proteinase B.

Authors:  G S Zubenko; A P Mitchell; E W Jones
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

6.  A temperature-sensitive dcw1 mutant of Saccharomyces cerevisiae is cell cycle arrested with small buds which have aberrant cell walls.

Authors:  Hiroshi Kitagaki; Kiyoshi Ito; Hitoshi Shimoi
Journal:  Eukaryot Cell       Date:  2004-10

7.  A recombinant Saccharomyces cerevisiae strain overproducing mannoproteins stabilizes wine against protein haze.

Authors:  Daniel Gonzalez-Ramos; Eduardo Cebollero; Ramon Gonzalez
Journal:  Appl Environ Microbiol       Date:  2008-07-07       Impact factor: 4.792

8.  Yeast Nap1-binding protein Nbp2p is required for mitotic growth at high temperatures and for cell wall integrity.

Authors:  Kentaro Ohkuni; Asuko Okuda; Akihiko Kikuchi
Journal:  Genetics       Date:  2003-10       Impact factor: 4.562

9.  The osmotic integrity of the yeast cell requires a functional PKC1 gene product.

Authors:  G Paravicini; M Cooper; L Friedli; D J Smith; J L Carpentier; L S Klig; M A Payton
Journal:  Mol Cell Biol       Date:  1992-11       Impact factor: 4.272

10.  Mapping of the proteinase b structural gene PRB1, in Saccharomyces cerevisiae and identification of nonsense alleles within the locus.

Authors:  G S Zubenko; A P Mitchell; E W Jones
Journal:  Genetics       Date:  1980-09       Impact factor: 4.562
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