Literature DB >> 3719998

Enzyme electrode for the measurement of lactate in undiluted blood.

W H Mullen, S J Churchouse, F H Keedy, P M Vadgama.   

Abstract

An enzyme electrode is described based upon the enzyme lactate oxidase (EC 1.1.3.2) coupled to an H2O2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l L-lactate with response times of 1-3 min, whilst giving independence from stirring. The method is suitable for the bedside analysis of a single drop of undiluted whole blood. Correlation with a routine method gave y = 0.96x + 0.11, r = 0.996, n = 46.

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Year:  1986        PMID: 3719998     DOI: 10.1016/0009-8981(86)90225-1

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN: 0009-8981            Impact factor:   3.786


  2 in total

Review 1.  Optimisation of enzyme electrodes.

Authors:  L X Tang; P Vadgama
Journal:  Med Biol Eng Comput       Date:  1990-05       Impact factor: 2.602

2.  Whole blood L-lactate assay by a new differential pH method: application to metabolic investigations.

Authors:  L Luzi; M Ripamonti; C Marconi; E Cazzola; A Mosca
Journal:  Acta Diabetol Lat       Date:  1990 Apr-Jun
  2 in total

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