Literature DB >> 371849

Separation of Plasmodium berghei-parasitized rat erythrocytes by means of carrier-free electrophoresis.

M Suzuki, Y Sawasaki, S Waki, H Iwaoka, T Asada, H Nakajima.   

Abstract

A carrier-free electrophoresis apparatus was used to isolate rat erythrocytes parasitized with Plasmodium berghei. The region of high electrophoretic mobility yielded noninfected erythrocytes, whereas that of low electrophoretic mobility yielded erythrocytes infected with viable parasites. Over 98% purity of parasitized erythrocytes was obtained when blood at an advanced stage of parasitaemia was used. Merozoite-rich fractions were also observed. This continuous one-step separation method should provide large numbers of parasitized erythrocytes for use in immunological and biochemical studies of malaria.

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Year:  1979        PMID： 371849      PMCID： PMC2395763     

Source DB:  PubMed          Journal:  Bull World Health Organ        ISSN： 0042-9686            Impact factor:   9.408


  7 in total

1.  [A NEW DEVELOPMENT FOR CONTINUOUS, CARRIER-FREE ELECTROPHORESIS. ON THE SEPARATION OF HIGH-MOLECULAR WEIGHT AND THICKLY DISPERSED PARTICLES].

Authors:  K HANNIG
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1964

2.  Change in electrophoretic mobility of human erythrocyte as the result of membrane shape change in vitro.

Authors:  T Sato; T Fujii; K Kojima
Journal:  Physiol Chem Phys       Date:  1975

3.  A comparison of methods used for the removal of white cells from malaria-infected blood.

Authors:  C A Homewood; K D Neame
Journal:  Ann Trop Med Parasitol       Date:  1976-06

4.  A merozoite vaccine effective against Plasmodium knowlesi malaria.

Authors:  G H Mitchell; G A Butcher; S Cohen
Journal:  Nature       Date:  1974-11-22       Impact factor: 49.962

5.  Culture of human malaria parasites Plasmodium falciparum.

Authors:  J D Haynes; C L Diggs; F A Hines; R E Desjardins
Journal:  Nature       Date:  1976-10-28       Impact factor: 49.962

6.  A new method for the preparation of rat liver lysosomes. Separation of cell organelles of rat liver by carrier-free continuous electrophoresis.

Authors:  R Stahn; K P Maier; K Hannig
Journal:  J Cell Biol       Date:  1970-09       Impact factor: 10.539

7.  Human malaria parasites in continuous culture.

Authors:  W Trager; J B Jensen
Journal:  Science       Date:  1976-08-20       Impact factor: 47.728
  7 in total
  2 in total

1.  Free-flow electrophoresis to clean donated blood before transfusion at the point of care: a proof-of-concept study.

Authors:  Michael C Larson
Journal:  Blood Transfus       Date:  2014-11-20       Impact factor: 3.443

2.  A simple method for separation of uninfected erythrocytes from those infected with Plasmodium berghei and for isolation of artificially released parasites.

Authors:  E A Nillni; M V Londner; D T Spira
Journal:  Z Parasitenkd       Date:  1981
  2 in total

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