| Literature DB >> 3717545 |
F A Anthony, D L Merat, W Y Cheung.
Abstract
A continuous spectrofluorimetric assay of calmodulin-dependent phosphatase is described. The assay monitors the formation of a fluorescent 4-methylumbelliferone from the dephosphorylation of 4-methylumbelliferyl phosphate and detects as little as 1 pmol of 4-methylumbelliferone. The phosphatase shows a Km of 1.3 mM for the substrate and a Vmax of 100 nmol/mg/min.Entities:
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Year: 1986 PMID： 3717545 DOI： 10.1016/0003-2697(86)90232-0
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365