| Literature DB >> 3714972 |
C Baumstark-Khan, H Rink, H P Zimmermann.
Abstract
Spindle formation and nuclear division of budding and irradiated yeast cells (Saccharomyces uvarum) was investigated by fluorescence microscopy of protoplasted cells. Protoplasts were treated with antitubulin antibodies and DAPI, a fluorescent dye staining DNA. In budding yeast cells, duplication of spindle pole bodies as well as formation of complete 1-micron spindles and elongated 8-micron spindles were documented. In X-irradiated cells, spindle pole bodies were duplicated as well, forming the complete 1-micron spindle. Nuclei of giant cells have lost the elongation ability and remain in a "normal" G2-phase state, thus preventing nuclear as well as cellular division.Entities:
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Year: 1986 PMID: 3714972 DOI: 10.1007/bf01209681
Source DB: PubMed Journal: Radiat Environ Biophys ISSN: 0301-634X Impact factor: 1.925