| Literature DB >> 3712439 |
A Yonath, M A Saper, I Makowski, J Müssig, J Piefke, H D Bartunik, K S Bartels, H G Wittmann.
Abstract
Single, three-dimensional crystals of the 50 S ribosomal subunit from Bacillus stearothermophilus (strain NCA) have been characterized using a synchrotron X-ray source. The crystals are orthorhombic with unit cell dimensions: a = 350 A, b = 670 A, c = 905 A, and contain at least one 2-fold screw axis. With cooling to -2 degrees C, the large crystals (1.0 mm X 0.2 mm X 0.1 mm) diffract to 15 to 18 A resolution and are stable in the synchrotron beam for several hours. Despite the large cell dimensions, the reflections are readily resolved when the X-ray diffraction patterns are densitometered with a 25 microns faster.Entities:
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Year: 1986 PMID： 3712439 DOI： 10.1016/0022-2836(86)90342-6
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469