Literature DB >> 3711181

Separation of normal and abnormal hemoglobin chains by reversed-phase high-performance liquid chromatography.

F Kutlar, A Kutlar, T H Huisman.   

Abstract

A review is presented of the elution patterns on reversed-phase columns of the normal and abnormal globin chains of different hemoglobin types, including 16 beta-chain variants, 7 alpha-chain variants, 9 gamma-chain variants, and 4 variants with fusion or hybrid chains. Separations appear to be based primarily on differences in hydrophobicity. The method is ideally suited for the detection of abnormal globin chains, their quantitation and their isolation. Semi-quantitative data based on the calculation of the delta/non-alpha ratios allow the detection of beta-thalassemic conditions in situations where the quantitation of hemoglobin A2 by other procedures is impossible or complicated.

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Year:  1986        PMID： 3711181     DOI： 10.1016/s0021-9673(01)95816-9

Source DB:  PubMed          Journal:  J Chromatogr


  18 in total

1.  Species identification of blood and bloodstains by high-performance liquid chromatography.

Authors:  H Inoue; F Takabe; O Takenaka; M Iwasa; Y Maeno
Journal:  Int J Legal Med       Date:  1990-12       Impact factor: 2.686

2.  Heterogeneity of the hemoglobin of the Ohrid trout (Salmo L. typicus).

Authors:  B Cepreganova; J B Wilson; B B Webber; B Kjovkareska; G D Efremov; T H Huisman
Journal:  Biochem Genet       Date:  1992-08       Impact factor: 1.890

3.  Fetal hemoglobin in normal adults and beta-thalassemia heterozygotes.

Authors:  A Kutlar; F Kutlar; L G Gu; S M Mayson; T H Huisman
Journal:  Hum Genet       Date:  1990-06       Impact factor: 4.132

4.  The linkage of Hb Valletta [alpha 2 beta 287(f3)Thr----Pro] and Hb F-Malta-I [alpha 2G gamma 2117(G19)His----Arg] in the Maltese population.

Authors:  F Kutlar; A E Felice; J L Grech; W H Bannister; A Kutlar; J B Wilson; B B Webber; H Y Hu; T H Huisman
Journal:  Hum Genet       Date:  1991-04       Impact factor: 4.132

5.  Identification of fetal hemoglobin and simultaneous estimation of bloodstain age by high-performance liquid chromatography.

Authors:  H Inoue; F Takabe; M Iwasa; Y Maeno
Journal:  Int J Legal Med       Date:  1991       Impact factor: 2.686

6.  Compound heterozygosity for a beta zero-thalassemia (frameshift codons 38/39; -C) and a nondeletional Swiss type of HPFH (A----C at NT -110, G gamma) in a Czechoslovakian family.

Authors:  K Indrak; J Indrakova; F Kutlar; D Pospisilova; I Sulovska; E Baysal; T H Huisman
Journal:  Ann Hematol       Date:  1991-08       Impact factor: 3.673

7.  A Czechoslovakian teenager with Hb E-beta zero-thalassemia [IVS-I-1 (G----A)] complicated by the presence of an alpha-globin gene triplication.

Authors:  K Indrak; Y J Fei; H W Li; E Baysal; V Brabec; H Fortova; J Cermak; T H Huisman
Journal:  Ann Hematol       Date:  1991-07       Impact factor: 3.673

8.  Beta S haplotypes in various world populations.

Authors:  C Oner; A J Dimovski; N F Olivieri; G Schiliro; J F Codrington; S Fattoum; A D Adekile; R Oner; G T Yüregir; C Altay
Journal:  Hum Genet       Date:  1992-04       Impact factor: 4.132

Review 9.  Hb F in sickle cell anemia.

Authors:  A D Adekile; T H Huisman
Journal:  Experientia       Date:  1993-01-15

10.  The in vivo expression of the globin genes of the beta cistron in gamma-, delta-, and delta beta-thalassemia heterozygotes.

Authors:  A J Dimovski; A D Adekile; T H Huisman
Journal:  Experientia       Date:  1994-02-15
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