| Literature DB >> 3709795 |
M A Grachev, G R Hartmann, T G Maximova, A A Mustaev, A R Schäffner, H Sieber, E F Zaychikov.
Abstract
DNA-dependent RNA polymerase B (II) from wheat germ was modified by incubation with 4-[N-(beta-hydroxyethyl)-N-methyl]benzaldehyde esters of AMP, ADP or ATP, followed by reduction with NaBH4. Reaction of the modified enzyme with [alpha-32P]UTP in the presence of various DNA templates led to a highly selective affinity labelling of the subunit with Mr 140 000 by covalently linked ApU. Labelling was inhibited by 1 microgram/ml alpha-amanitin.Entities:
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Year: 1986 PMID： 3709795 DOI： 10.1016/0014-5793(86)81154-1
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124