How is ventilation maintained in the presence of the muscle relaxant, dantrolene sodium? A study in the anaesthetized rat.

The effects of intravenous injection of the muscle relaxant dantrolene sodium on ventilation, integrated EMG of external intercostal muscles and motor unit discharge in these muscles were measured in anaesthetized rats. Dantrolene sodium was administered in a carrier solution at pH 10. The carrier was not responsible for the effects seen on dantrolene sodium infusion. Dantrolene sodium (5 mg kg-1 body weight) reduced inspired ventilation (VI) on average from 308 +/- 106 ml min-1 (s.d., n = 8) in the control period to 247 +/- 118 ml min-1 (n = 8) after dantrolene sodium. Expired ventilation was reduced likewise from 274 +/- 132 ml min-1 (n = 8) to 229 +/- 109 ml min-1 (n = 8). These changes are significant at P less than 0.05. The integrated EMG was increased by 40.0 +/- 29.3% (n = 9), a change significant at P less than 0.001. Dantrolene sodium not only decreased the mean interspike interval of individual motor units by 16.2 +/- 11.1% (n = 13), a change significant at P less than 0.001, but also increased the duration of the burst of activity of each individual motor unit on average by 36.2 +/- 52.4% (n = 13), a change significant at P less than 0.05. The recruitment of additional motor units was also effected by dantrolene sodium. These units did not behave in any noticeably different manner from units previously active. It is concluded that the nervous system compensates for the effect of dantrolene sodium only to a limited extent by increasing the frequency of discharge of active motor units. Recruitment ofadditional motor units and increased burst duration play at least as important a role.