Literature DB >> 3706733

Rapid fractionation of collagen chains and peptides by high-performance liquid chromatography.

J F Bateman, T Mascara, D Chan, W G Cole.   

Abstract

A strategy was developed, using a Pharmacia Fast Protein Liquid chromatography (FPLC) system, for the rapid preparation of the alpha-chains, cyanogen bromide peptides and tryptic peptides of type I collagen obtained from tissues and cultured fibroblasts. Collagen alpha-chains were prepared using a C18 PEP-RPC reverse-phase column and volatile solvents. Preliminary Superose 6 gel permeation chromatography was used to separate the crosslinked beta- and gamma-chains from the alpha-chains of tissue collagen samples. A Mono S cation-exchange column was used to resolve all of the major type I collagen cyanogen bromide peptides including the alpha 1(I)CB7 and CB8 peptides, which have not been well resolved by previously published methods. Collagen tryptic peptides were chromatographed on the PEP-RPC reverse-phase column.

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Year:  1986        PMID： 3706733     DOI： 10.1016/0003-2697(86)90534-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

1.  Lysyl hydroxylation in collagens from hyperplastic callus and embryonic bones.

Authors:  H W Lehmann; M Bodo; C Frohn; A Nerlich; D Rimek; H Notbohm; P K Müller
Journal:  Biochem J       Date:  1992-03-01       Impact factor: 3.857

2.  Collagen defects in lethal perinatal osteogenesis imperfecta.

Authors:  J F Bateman; D Chan; T Mascara; J G Rogers; W G Cole
Journal:  Biochem J       Date:  1986-12-15       Impact factor: 3.857

3.  Mutations That Alter the Carboxy-Terminal-Propeptide Cleavage Site of the Chains of Type I Procollagen Are Associated With a Unique Osteogenesis Imperfecta Phenotype.

Authors:  Tim Cundy; Michael Dray; John Delahunt; Jannie Dahl Hald; Bente Langdahl; Chumei Li; Marta Szybowska; Shehla Mohammed; Emma L Duncan; Aideen M McInerney-Leo; Patricia G Wheeler; Paul Roschger; Klaus Klaushofer; Jyoti Rai; MaryAnn Weis; David Eyre; Ulrike Schwarze; Peter H Byers
Journal:  J Bone Miner Res       Date:  2018-04-18       Impact factor: 6.741

4.  Expression, in cartilage, of a 7-amino-acid deletion in type II collagen from two unrelated individuals with Kniest dysplasia.

Authors:  R Bogaert; D Wilkin; W R Wilcox; R Lachman; D Rimoin; D H Cohn; D R Eyre
Journal:  Am J Hum Genet       Date:  1994-12       Impact factor: 11.025

5.  Fulvic acid supplementation and selenium deficiency disturb the structural integrity of mouse skeletal tissue. An animal model to study the molecular defects of Kashin-Beck disease.

Authors:  C Yang; C Niu; M Bodo; E Gabriel; H Notbohm; E Wolf; P K Müller
Journal:  Biochem J       Date:  1993-02-01       Impact factor: 3.857

6.  Cartilage expression of a type II collagen mutation in an inherited form of osteoarthritis associated with a mild chondrodysplasia.

Authors:  D R Eyre; M A Weis; R W Moskowitz
Journal:  J Clin Invest       Date:  1991-01       Impact factor: 14.808

7.  Analysis of the age-related composition of human skin collagen and collagens synthesized by fibroblast culture.

Authors:  J Brinckmann; M Bodo; M Brey; H H Wolff; P K Müller
Journal:  Arch Dermatol Res       Date:  1994       Impact factor: 3.017

8.  Biochemical analysis of callus tissue in osteogenesis imperfecta type IV. Evidence for transient overmodification in collagen types I and III.

Authors:  R E Brenner; U Vetter; A Nerlich; O Wörsdorfer; W M Teller; P K Müller
Journal:  J Clin Invest       Date:  1989-09       Impact factor: 14.808

9.  Analysis of alpha 1 beta 1, alpha 2 beta 1 and alpha 3 beta 1 integrins in cell--collagen interactions: identification of conformation dependent alpha 1 beta 1 binding sites in collagen type I.

Authors:  D Gullberg; K R Gehlsen; D C Turner; K Ahlén; L S Zijenah; M J Barnes; K Rubin
Journal:  EMBO J       Date:  1992-11       Impact factor: 11.598
  9 in total

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