| Literature DB >> 3706711 |
Abstract
Interest in accurate measurement of biotin concentrations in plasma and urine has been stimulated by recent advances in the understanding of biotin-responsive inborn errors of metabolism and by several reports describing acquired biotin deficiency during parenteral alimentation. This paper presents a biotin assay utilizing radiolabeled avidin in a sequential, solid-phase method; the assay has increased sensitivity compared to previous methods (greater than or equal to 10 fmol/tube), correlates with expected trends in biotin concentrations in blood and urine in a rat model of biotin deficiency, and can utilize commercially available radiolabeled avidin.Entities:
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Year: 1986 PMID: 3706711 DOI: 10.1016/0003-2697(86)90092-8
Source DB: PubMed Journal: Anal Biochem ISSN: 0003-2697 Impact factor: 3.365