| Literature DB >> 3706706 |
D R Kuonen, P J Roberts, I R Cottingham.
Abstract
A nondenaturing gradient polyacrylamide gel electrophoresis method is described for the resolution of membrane proteins. Bovine heart inner mitochondrial membranes were solubilized in Triton X-100 and individual complexes were identified by staining for activity and protein. Succinate dehydrogenase was isolated by band excision and shown by electrophoresis under denaturing conditions to be highly purified. In addition, the electrophoretic transfer of NADH dehydrogenase to nitrocellulose was demonstrated. The enzyme was identified on the resulting blot by activity staining and the binding of monospecific antibodies.Entities:
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Year: 1986 PMID： 3706706 DOI： 10.1016/0003-2697(86)90084-9
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365