Literature DB >> 3704639

Protein, DNA, and virus crystallography with a focused imaging proportional counter.

R M Durbin, R Burns, J Moulai, P Metcalf, D Freymann, M Blum, J E Anderson, S C Harrison, D C Wiley.   

Abstract

A set of programs has been developed for rapid collection of x-ray intensity data from protein and virus crystals with a commercially available two-dimensional focused geometry electronic detector. The detector is compact and portable, with unusually high spatial resolution comparable to that used in oscillation photography. It has allowed x-ray data collection on weakly diffracting crystals with large unit cells, as well as more conventional "diffractometer-quality" crystals. The quality of the data is compared with that from oscillation photography and automated diffractometry in the range of unit cells from 96.3 to 383.2 angstroms. Isomorphous and anomalous difference Pattersons, based on detector data, are shown for a variable surface glycoprotein mercury derivative and for a repressor-DNA bromine derivative, which has been solved at 7 angstroms with detector data only.

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Year:  1986        PMID： 3704639     DOI： 10.1126/science.3704639

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  7 in total

1.  Molecular structure of leucine aminopeptidase at 2.7-A resolution.

Authors:  S K Burley; P R David; A Taylor; W N Lipscomb
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

2.  Crystallographic detection of a second ligand binding site in influenza virus hemagglutinin.

Authors:  N K Sauter; G D Glick; R L Crowther; S J Park; M B Eisen; J J Skehel; J R Knowles; D C Wiley
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

3.  Rat acid phosphatase: overexpression of active, secreted enzyme by recombinant baculovirus-infected insect cells, molecular properties, and crystallization.

Authors:  P Vihko; R Kurkela; K Porvari; A Herrala; A Lindfors; Y Lindqvist; G Schneider
Journal:  Proc Natl Acad Sci U S A       Date:  1993-02-01       Impact factor: 11.205

4.  Purification and characterization of Klebsiella aerogenes UreE protein: a nickel-binding protein that functions in urease metallocenter assembly.

Authors:  M H Lee; H S Pankratz; S Wang; R A Scott; M G Finnegan; M K Johnson; J A Ippolito; D W Christianson; R P Hausinger
Journal:  Protein Sci       Date:  1993-06       Impact factor: 6.725

5.  Structure-assisted redesign of a protein-zinc-binding site with femtomolar affinity.

Authors:  J A Ippolito; T T Baird; S A McGee; D W Christianson; C A Fierke
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-23       Impact factor: 11.205

6.  Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis.

Authors:  E F Pai; U Krengel; G A Petsko; R S Goody; W Kabsch; A Wittinghofer
Journal:  EMBO J       Date:  1990-08       Impact factor: 11.598

7.  The three-dimensional structure of P2 myelin protein.

Authors:  T A Jones; T Bergfors; J Sedzik; T Unge
Journal:  EMBO J       Date:  1988-06       Impact factor: 11.598
  7 in total

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