Literature DB >> 3703676

Effect of trypsinization and histone H5 addition on DNA twist and topology in reconstituted minichromosomes.

R H Morse, C R Cantor.   

Abstract

Free DNA in solution exhibits an untwisting of the double helix with increasing temperature. We have shown previously that when DNA is reconstituted with histones to form nucleosome core particles, both the core DNA and the adjacent linker DNA are constrained from thermal untwisting. The origin of this constraint is unknown. Here we examine the effect of two modifications of nucleosome structure on the constraint against thermal untwisting, and also on DNA topology. In one experiment, we removed the highly positively charged histone amino and carboxy termini by trypsinization. Alternatively, we added histone H5, a histone H1 variant from chick erythrocytes. Neither of these modifications had any major effect on DNA topology or twist in the nucleosome.

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Year:  1986        PMID： 3703676      PMCID： PMC339769          DOI： 10.1093/nar/14.8.3293

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  34 in total

1.  Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors:  T Maniatis; A Jeffrey; H van deSande
Journal:  Biochemistry       Date:  1975-08-26       Impact factor: 3.162

2.  Folding of the DNA double helix in chromatin-like structures from simian virus 40.

Authors:  J E Germond; B Hirt; P Oudet; M Gross-Bellark; P Chambon
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

3.  Conformational fluctuations of DNA helix.

Authors:  D E Depew; J C Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

4.  Localization of the sites along nucleosome DNA which interact with NH2-terminal histone regions.

Authors:  J P Whitlock; R T Simpson
Journal:  J Biol Chem       Date:  1977-09-25       Impact factor: 5.157

5.  Chromatin core particle unfolding induced by tryptic cleavage of histones.

Authors:  D M Lilley; K Tatchell
Journal:  Nucleic Acids Res       Date:  1977-06       Impact factor: 16.971

6.  Dissection of chromosome structure with trypsin and nucleases.

Authors:  H Weintraub; F Van Lente
Journal:  Proc Natl Acad Sci U S A       Date:  1974-10       Impact factor: 11.205

7.  Folding of DNA by histones which lack their NH2-terminal regions.

Authors:  J P Whitlock; A Stein
Journal:  J Biol Chem       Date:  1978-06-10       Impact factor: 5.157

8.  Effects of DNA binding proteins on DNA methylation in vitro.

Authors:  T L Kautiainen; P A Jones
Journal:  Biochemistry       Date:  1985-02-26       Impact factor: 3.162

9.  A modified procedure for fractionating histones.

Authors:  D Oliver; K R Sommer; S Panyim; S Spiker; R Chalkley
Journal:  Biochem J       Date:  1972-09       Impact factor: 3.857

10.  H5 Histone and DNA-relaxing enzyme of chicken erythrocytes. Interaction with superhelical DNA.

Authors:  M Bina-Stein; T Vogel; D S Singer; M F Singer
Journal:  J Biol Chem       Date:  1976-12-10       Impact factor: 5.157
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  13 in total

1.  Effects of histone acetylation on chromatin topology in vivo.

Authors:  L C Lutter; L Judis; R F Paretti
Journal:  Mol Cell Biol       Date:  1992-11       Impact factor: 4.272

2.  Histone contributions to the structure of DNA in the nucleosome.

Authors:  J J Hayes; D J Clark; A P Wolffe
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-01       Impact factor: 11.205

3.  Influence of DNA topology and histone tails in nucleosome organization on pBR322 DNA.

Authors:  M Buttinelli; L Leoni; B Sampaolese; M Savino
Journal:  Nucleic Acids Res       Date:  1991-08-25       Impact factor: 16.971

4.  Reconstitution of hyperacetylated, DNase I-sensitive chromatin characterized by high conformational flexibility of nucleosomal DNA.

Authors:  W A Krajewski; P B Becker
Journal:  Proc Natl Acad Sci U S A       Date:  1998-02-17       Impact factor: 11.205

5.  Interaction of the histone (H3-H4)2 tetramer of the nucleosome with positively supercoiled DNA minicircles: Potential flipping of the protein from a left- to a right-handed superhelical form.

Authors:  A Hamiche; V Carot; M Alilat; F De Lucia; M F O'Donohue; B Revet; A Prunell
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-23       Impact factor: 11.205

6.  A topological approach to nucleosome structure and dynamics: the linking number paradox and other issues.

Authors:  A Prunell
Journal:  Biophys J       Date:  1998-05       Impact factor: 4.033

Review 7.  DNA-protein interactions and spatial organization of DNA.

Authors:  W A Krajewski; S V Razin
Journal:  Mol Biol Rep       Date:  1993-10       Impact factor: 2.316

8.  Supercoiling in prokaryotic and eukaryotic DNA: changes in response to topological perturbation of plasmids in E. coli and SV40 in vitro, in nuclei and in CV-1 cells.

Authors:  F Esposito; R R Sinden
Journal:  Nucleic Acids Res       Date:  1987-07-10       Impact factor: 16.971

9.  Thermal unwinding of simian virus 40 transcription complex DNA.

Authors:  L C Lutter
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

10.  Histone N-terminal tails interfere with nucleosome traversal by RNA polymerase II.

Authors:  Andrea Ujvári; Fu-Kai Hsieh; Susan W Luse; Vasily M Studitsky; Donal S Luse
Journal:  J Biol Chem       Date:  2008-09-23       Impact factor: 5.157
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