Literature DB >> 370328

A method for isolating uncontaminated nuclei from all stages of developing Xenopus laevis embryos.

F Farzaneh, C K Pearson.   

Abstract

A method for isolating nuclei from Xenopus laevis embryos has been developed. This procedure enables the isolation of nuclei, free from contamination with yolk and pigment granules, at all stages of embryoic development. Using this method the nuclear yield is 60--70% of the estimated number of cells in the embryo. The DNA, RNA, histone and non-histone protein content of these nuclei during embryogenesis (from early cleavage to the swimming tadpole stage) has been measured.

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Year:  1978        PMID： 370328

Source DB:  PubMed          Journal:  J Embryol Exp Morphol        ISSN： 0022-0752


  8 in total

1.  Translocation of a store of maternal cytoplasmic c-myc protein into nuclei during early development.

Authors:  M Gusse; J Ghysdael; G Evan; T Soussi; M Méchali
Journal:  Mol Cell Biol       Date:  1989-12       Impact factor: 4.272

2.  Neural-inducing activity of nuclei and nuclear fractions from Xenopus laevis embryos.

Authors:  Günther Bretzel; Hildegard Tiedemann
Journal:  Rouxs Arch Dev Biol       Date:  1986-02

3.  DNase-seq to Study Chromatin Accessibility in Early Xenopus tropicalis Embryos.

Authors:  Jin Sun Cho; Ira L Blitz; Ken W Y Cho
Journal:  Cold Spring Harb Protoc       Date:  2019-04-01

4.  A quantitative analysis of signal transduction from activin receptor to nucleus and its relevance to morphogen gradient interpretation.

Authors:  K Shimizu; J B Gurdon
Journal:  Proc Natl Acad Sci U S A       Date:  1999-06-08       Impact factor: 11.205

5.  The distribution of nucleoplasmin in early development and organogenesis of Xenopus laevis.

Authors:  D Wedlich; C Dreyer
Journal:  Cell Tissue Res       Date:  1988-11       Impact factor: 5.249

6.  Replication forks are underrepresented in chromosomal DNA of Xenopus laevis embryos.

Authors:  M F Gaudette; R M Benbow
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

7.  Selective and rapid nuclear translocation of a c-Myc-containing complex after fertilization of Xenopus laevis eggs.

Authors:  J M Lemaitre; S Bocquet; R Buckle; M Mechali
Journal:  Mol Cell Biol       Date:  1995-09       Impact factor: 4.272

8.  Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.

Authors:  M J Lohka; J L Maller
Journal:  J Cell Biol       Date:  1985-08       Impact factor: 10.539
  8 in total

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