Literature DB >> 3701846

Rat heart gap junctions as disulfide-bonded connexon multimers: their depolymerization and solubilization in deoxycholate.

C K Manjunath, E Page.   

Abstract

Unproteolyzed gap junctions isolated from rat heart and liver were analyzed for the presence of inter-subunit disulfide bonds by sodium dodecylsulfate polyacrylamide gel electrophoresis. Rat cardiac junctions contained multiple disulfide bonds connecting the Mr 47,000 subunits of the same connexon and of different connexons. Inter-subunit disulfide bonds were absent in liver junctions. Unproteolyzed rat heart gap junctions were resistant to deoxycholate in their oxidized state, but dissolved readily in the detergent when the disulfide bonds were cleaved with beta-mercaptoethanol. Disulfide bonding in proteolyzed cardiac junctions was limited to pairs of Mr 29,500 subunits. These junctions were not soluble in deoxycholate even in the presence of beta-mercaptoethanol. These results show that heart and liver junctions differ in their quarternary organization.

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Year:  1986        PMID： 3701846     DOI： 10.1007/bf01869685

Source DB:  PubMed          Journal:  J Membr Biol        ISSN： 0022-2631            Impact factor:   1.843


  35 in total

1.  Lens membranes II. Isolation and characterization of the main intrinsic polypeptide (MIP) of bovine lens fiber membranes.

Authors:  R M Broekhuyse; E D Kuhlmann; A L Stols
Journal:  Exp Eye Res       Date:  1976-09       Impact factor: 3.467

2.  A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.

Authors:  M A Markwell; S M Haas; L L Bieber; N E Tolbert
Journal:  Anal Biochem       Date:  1978-06-15       Impact factor: 3.365

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Lens gap junctions: a structural hypothesis for nonregulated low-resistance intercellular pathways.

Authors:  D A Goodenough
Journal:  Invest Ophthalmol Vis Sci       Date:  1979-11       Impact factor: 4.799

Review 5.  Junctional intercellular communication: the cell-to-cell membrane channel.

Authors:  W R Loewenstein
Journal:  Physiol Rev       Date:  1981-10       Impact factor: 37.312

6.  Effect of intracellular injection of cAMP on the electrical coupling of mammalian cardiac cells.

Authors:  W C De Mello
Journal:  Biochem Biophys Res Commun       Date:  1984-03-30       Impact factor: 3.575

7.  Hormonal regulation of cell junction permeability: upregulation by catecholamine and prostaglandin E1.

Authors:  A Radu; G Dahl; W R Loewenstein
Journal:  J Membr Biol       Date:  1982       Impact factor: 1.843

8.  Cell junction and cycle AMP: III. Promotion of junctional membrane permeability and junctional membrane particles in a junction-deficient cell type.

Authors:  R Azarnia; G Dahl; W R Loewenstein
Journal:  J Membr Biol       Date:  1981       Impact factor: 1.843

9.  Isolation and protein composition of gap junctions from rabbit hearts.

Authors:  C K Manjunath; G E Goings; E Page
Journal:  Biochem J       Date:  1982-07-01       Impact factor: 3.857

10.  Bulk isolation of mouse hepatocyte gap junctions. Characterization of the principal protein, connexin.

Authors:  D A Goodenough
Journal:  J Cell Biol       Date:  1974-05       Impact factor: 10.539
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  10 in total

1.  Tissue-specific distribution of differentially phosphorylated forms of Cx43.

Authors:  R Kadle; J T Zhang; B J Nicholson
Journal:  Mol Cell Biol       Date:  1991-01       Impact factor: 4.272

2.  Cross-linking of cardiac gap junction connexons by thiol/disulfide exchanges.

Authors:  E Dupont; A el Aoumari; J P Briand; C Fromaget; D Gros
Journal:  J Membr Biol       Date:  1989-06       Impact factor: 1.843

3.  Immunological characterization of rat cardiac gap junctions: presence of common antigenic determinants in heart of other vertebrate species and in various organs.

Authors:  E Dupont; A el Aoumari; S Roustiau-Sévère; J P Briand; D Gros
Journal:  J Membr Biol       Date:  1988-09       Impact factor: 1.843

4.  Turnover and phosphorylation dynamics of connexin43 gap junction protein in cultured cardiac myocytes.

Authors:  D W Laird; K L Puranam; J P Revel
Journal:  Biochem J       Date:  1991-01-01       Impact factor: 3.857

5.  Attempts to define functional domains of gap junction proteins with synthetic peptides.

Authors:  G Dahl; W Nonner; R Werner
Journal:  Biophys J       Date:  1994-11       Impact factor: 4.033

6.  Isolation and purification of gap junction channels.

Authors:  K A Stauffer; N M Kumar; N B Gilula; N Unwin
Journal:  J Cell Biol       Date:  1991-10       Impact factor: 10.539

7.  The 43-kD polypeptide of heart gap junctions: immunolocalization, topology, and functional domains.

Authors:  S B Yancey; S A John; R Lal; B J Austin; J P Revel
Journal:  J Cell Biol       Date:  1989-06       Impact factor: 10.539

8.  Modulation of gap junction transcript and protein expression during pregnancy in the rat.

Authors:  B Risek; S Guthrie; N Kumar; N B Gilula
Journal:  J Cell Biol       Date:  1990-02       Impact factor: 10.539

9.  Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques.

Authors:  L S Musil; D A Goodenough
Journal:  J Cell Biol       Date:  1991-12       Impact factor: 10.539

10.  Reconstitution of native-type noncrystalline lens fiber gap junctions from isolated hemichannels.

Authors:  J Kistler; K Goldie; P Donaldson; A Engel
Journal:  J Cell Biol       Date:  1994-08       Impact factor: 10.539
  10 in total

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