Literature DB >> 3701070

Rat monoclonal antibodies. VI. Production of IgA secreting hybridomas with specificity for the 2,4-dinitrophenyl (DNP) hapten.

M Rits, F Cormont, H Bazin, R Meykens, J P Vaerman.   

Abstract

A simple method to obtain rat hybridomas producing specific IgA antibodies is reported. By fusing the IR983F rat myeloma cell line with mesenteric lymph node cells from LOU/C rats immunized via the Peyer's patches with DNP-Salmonella typhimurium, twenty hybrids secreting monoclonal IgA antibodies specific for DNP were produced and maintained as highly secreting transplantable ascitic tumors. The monoclonal IgA antibodies were easily purified by affinity chromatography on a DNP-immunosorbent and were found to comprise both monomers and polymers.

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Year:  1986        PMID： 3701070     DOI： 10.1016/0022-1759(86)90034-7

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  7 in total

1.  Clearance kinetics and tissue distribution of aggregated human serum IgA in rats.

Authors:  W M Bogers; A Gorter; M E Stuurman; L A Van Es; M R Daha
Journal:  Immunology       Date:  1989-06       Impact factor: 7.397

2.  The polymeric immunoglobulin receptor (secretory component) mediates transport of immune complexes across epithelial cells: a local defense function for IgA.

Authors:  C S Kaetzel; J K Robinson; K R Chintalacharuvu; J P Vaerman; M E Lamm
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-01       Impact factor: 11.205

3.  An acute model for IgA-mediated glomerular inflammation in rats induced by monoclonal polymeric rat IgA antibodies.

Authors:  R K Stad; J A Bruijn; D J van Gijlswijk-Janssen; L A van Es; M R Daha
Journal:  Clin Exp Immunol       Date:  1993-06       Impact factor: 4.330

4.  In vivo activation of complement by IgA in a rat model.

Authors:  R K Stad; W M Bogers; M E Thoomes-van der Sluys; L A Van Es; M R Daha
Journal:  Clin Exp Immunol       Date:  1992-01       Impact factor: 4.330

5.  Chemotactic properties of rat immunoglobulins and immune complexes.

Authors:  M Arashi; Y Sibille; W W Merrill; M Rits; H Bazin; J P Vaerman
Journal:  Infect Immun       Date:  1989-02       Impact factor: 3.441

6.  Binding of human IgA1 to rat peritoneal macrophages.

Authors:  A Gorter; P S Hiemstra; E A van der Voort; L A van Es; M R Daha
Journal:  Immunology       Date:  1988-06       Impact factor: 7.397

7.  Binding and transepithelial transport of immunoglobulins by intestinal M cells: demonstration using monoclonal IgA antibodies against enteric viral proteins.

Authors:  R Weltzin; P Lucia-Jandris; P Michetti; B N Fields; J P Kraehenbuhl; M R Neutra
Journal:  J Cell Biol       Date:  1989-05       Impact factor: 10.539
  7 in total

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