Literature DB >> 3700630

Separation of cryptosporidium oocysts from fecal debris by density gradient centrifugation and glass bead columns.

M B Heyman, L K Shigekuni, A J Ammann.   

Abstract

A method was developed to obtain purified cryptosporidium oocysts from fecal samples. Oocysts were initially collected by centrifugation through a sucrose density gradient and further purified by passage through glass bead columns. The purified oocysts were antigenically active and sufficiently pure for immunological studies.

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Year:  1986        PMID： 3700630      PMCID： PMC362839          DOI： 10.1128/jcm.23.4.789-791.1986

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

1.  A cleaning method for coccidial oocysts using density-gradient sedimentation.

Authors:  N N SHARMA; W M REID; J W FOSTER
Journal:  J Parasitol       Date:  1963-02       Impact factor: 1.276

2.  Separation of nematode eggs from fecal debris by gradient centrifugation.

Authors:  W C MARQUARDT
Journal:  J Parasitol       Date:  1961-04       Impact factor: 1.276

3.  Purification of Eimeria tenella sporozoites with glass bead columns.

Authors:  G E Wagenbach
Journal:  J Parasitol       Date:  1969-08       Impact factor: 1.276

4.  Experimental cryptosporidiosis in laboratory mice.

Authors:  D Sherwood; K W Angus; D R Snodgrass; S Tzipori
Journal:  Infect Immun       Date:  1982-11       Impact factor: 3.441

5.  The species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) infecting mammals.

Authors:  S J Upton; W L Current
Journal:  J Parasitol       Date:  1985-10       Impact factor: 1.276

6.  Techniques for the recovery and identification of Cryptosporidium oocysts from stool specimens.

Authors:  L S Garcia; D A Bruckner; T C Brewer; R Y Shimizu
Journal:  J Clin Microbiol       Date:  1983-07       Impact factor: 5.948
  6 in total
  8 in total

Review 1.  Cryptosporidium spp. and cryptosporidiosis.

Authors:  R Fayer; B L Ungar
Journal:  Microbiol Rev       Date:  1986-12

2.  Cryptosporidiosis: multiattribute evaluation of six diagnostic methods.

Authors:  D W MacPherson; R McQueen
Journal:  J Clin Microbiol       Date:  1993-02       Impact factor: 5.948

3.  Comparative evaluation of several techniques for purification of Cryptosporidium parvum oocysts from rat feces.

Authors:  P Suresh; J E Rehg
Journal:  J Clin Microbiol       Date:  1996-01       Impact factor: 5.948

4.  Effects of Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in mice.

Authors:  Valeria F Del Coco; Mónica D Sparo; Alicia Sidoti; Mónica Santín; Juan Angel Basualdo; María Alejandra Córdoba
Journal:  Parasitol Res       Date:  2016-05-19       Impact factor: 2.289

5.  Detection of Cryptosporidium parvum oocysts in bovine feces by monoclonal antibody capture enzyme-linked immunosorbent assay.

Authors:  K Z Anusz; P H Mason; M W Riggs; L E Perryman
Journal:  J Clin Microbiol       Date:  1990-12       Impact factor: 5.948

6.  Revisiting the infectivity and pathogenicity of Cryptosporidium avium provides new information on parasitic sites within the host.

Authors:  Zhaohui Cui; Dan Song; Meng Qi; Sumei Zhang; Rongjun Wang; Fuchun Jian; Changshen Ning; Longxian Zhang
Journal:  Parasit Vectors       Date:  2018-09-19       Impact factor: 3.876

7.  DNA extraction from protozoan oocysts/cysts in feces for diagnostic PCR.

Authors:  Yousry Hawash
Journal:  Korean J Parasitol       Date:  2014-06-26       Impact factor: 1.341

Review 8.  Cryptosporidiosis in perspective.

Authors:  S Tzipori
Journal:  Adv Parasitol       Date:  1988       Impact factor: 3.870
  8 in total

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