Literature DB >> 3700624

Radiometric method for the rapid detection of Leptospira organisms.

N Manca, R Verardi, D Colombrita, G Ravizzola, E Savoldi, A Turano.   

Abstract

A rapid and sensitive radiometric method for detection of Leptospira interrogans serovar pomona and Leptospira interrogans serovar copenhageni is described. Stuart's medium and Middlebrook TB (12A) medium supplemented with bovine serum albumin, catalase, and casein hydrolysate and labeled with 14C-fatty acids were used. The radioactivity was measured in a BACTEC 460 (Johnston Laboratories). With this system, Leptospira organisms were detected in human blood in 2 to 5 days, a notably shorter time period than that required for the majority of detection techniques.

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Year:  1986        PMID： 3700624      PMCID： PMC268657          DOI： 10.1128/jcm.23.2.401-403.1986

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  NUTRITION OF LEPTOSPIRA POMONA AND GROWTH OF 13 OTHER SEROTYPES: FRACTIONATION OF OLEIC ALBUMIN COMPLEX AND A MEDIUM OF BOVINE ALBUMIN AND POLYSORBATE 80.

Authors:  H C ELLINGHAUSEN; W G MCCULLOUGH
Journal:  Am J Vet Res       Date:  1965-01       Impact factor: 1.156

2.  Studies of technics for the isolation of leptospires. I. The suitability of 1 to 2 day old baby chicks as compared to hamsters for the isolation of various leptospiral serotypes.

Authors:  G W FISCHER; D K POWERS; M M GALTON
Journal:  J Infect Dis       Date:  1958 Sep-Oct       Impact factor: 5.226

3.  Characterization of leptospires according to fatty acid requirements.

Authors:  R C Johnson; V G Harris; J K Walby
Journal:  J Gen Microbiol       Date:  1969-03

4.  Early detection of bacterial growth, with carbon-14-labeled glucose.

Authors:  F H DeLand; H N Wagner
Journal:  Radiology       Date:  1969-01       Impact factor: 11.105

5.  Radiometric estimation of the replication time of bacteria in culture: an objective and precise approach to quantitative microbiology.

Authors:  E U Buddemeyer; G M Wells; R Hutchinson; M D Cooper; G S Johnston
Journal:  J Nucl Med       Date:  1978-06       Impact factor: 10.057
  5 in total
  1 in total

Review 1.  Leptospirosis.

Authors:  P N Levett
Journal:  Clin Microbiol Rev       Date:  2001-04       Impact factor: 26.132
  1 in total

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