Literature DB >> 3700603

Method for the typing of Clostridium difficile based on polyacrylamide gel electrophoresis of [35S]methionine-labeled proteins.

S Tabaqchali, S O'Farrell, D Holland, R Silman.   

Abstract

A typing method for Clostridium difficile based on the incorporation of [35S]methionine into cellular proteins, their separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their visualization by autoradiography is described. On analysis of the radiolabeled-protein profiles, nine distinct groups were observed (A to E and W to Z). The method, which is simple, reproducible, and readily expandable, has been applied in epidemiological studies to demonstrate cross-infection and hospital acquisition of C. difficile.

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Year:  1986        PMID： 3700603      PMCID： PMC268603          DOI： 10.1128/jcm.23.1.197-198.1986

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Isolation of Clostridium difficile from patients and the environment of hospital wards.

Authors:  H Malamou-Ladas; S O'Farrell; J Q Nash; S Tabaqchali
Journal:  J Clin Pathol       Date:  1983-01       Impact factor: 3.411

2.  Outbreaks of diarrhea associated with Clostridium difficile and its toxin in day-care centers: evidence of person-to-person spread.

Authors:  K Kim; H L DuPont; L K Pickering
Journal:  J Pediatr       Date:  1983-03       Impact factor: 4.406

3.  Investigation of an outbreak of antibiotic-associated colitis by various typing methods.

Authors:  J Wüst; N M Sullivan; U Hardegger; T D Wilkins
Journal:  J Clin Microbiol       Date:  1982-12       Impact factor: 5.948

4.  Isolation of Clostridium difficile from the feces and the antibody in sera of young and elderly adults.

Authors:  S Nakamura; M Mikawa; S Nakashio; M Takabatake; I Okado; K Yamakawa; T Serikawa; S Okumura; S Nishida
Journal:  Microbiol Immunol       Date:  1981       Impact factor: 1.955

5.  Epidemiology of Clostridium difficile in infants.

Authors:  H E Larson; F E Barclay; P Honour; I D Hill
Journal:  J Infect Dis       Date:  1982-12       Impact factor: 5.226

6.  Serogrouping of Clostridium difficile strains by slide agglutination.

Authors:  M Delmee; M Homel; G Wauters
Journal:  J Clin Microbiol       Date:  1985-03       Impact factor: 5.948

7.  Agglutination, toxigenicity and sorbitol fermentation of Clostridium difficile.

Authors:  S Nakamura; T Serikawa; M Mikawa; S Nakashio; K Yamakawa; S Nishida
Journal:  Microbiol Immunol       Date:  1981       Impact factor: 1.955

Review 8.  Antibiotic-associated pseudomembranous colitis.

Authors:  J G Bartlett
Journal:  Rev Infect Dis       Date:  1979 May-Jun

9.  Isolation of Clostridium difficile from the environment and contacts of patients with antibiotic-associated colitis.

Authors:  K H Kim; R Fekety; D H Batts; D Brown; M Cudmore; J Silva; D Waters
Journal:  J Infect Dis       Date:  1981-01       Impact factor: 5.226

10.  Clostridium difficile and the aetiology of pseudomembranous colitis.

Authors:  H E Larson; A B Price; P Honour; S P Borriello
Journal:  Lancet       Date:  1978-05-20       Impact factor: 79.321
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  20 in total

1.  Restriction endonuclease analysis of nosocomial isolates of Clostridium difficile.

Authors:  H R Devlin; W Au; L Foux; W C Bradbury
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

2.  Restriction endonuclease DNA analysis of Clostridium difficile.

Authors:  B W Wren; S Tabaqchali
Journal:  J Clin Microbiol       Date:  1987-12       Impact factor: 5.948

3.  Use of pulsed-field gel electrophoresis for investigation of an outbreak of Clostridium difficile infection among geriatric patients.

Authors:  D Talon; P Bailly; M Delmée; M Thouverez; B Mulin; M Iehl-Robert; V Cailleaux; Y Michel-Briand
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-11       Impact factor: 3.267

4.  Immunoblotting to demonstrate antigenic and immunogenic differences among nine standard strains of Clostridium difficile.

Authors:  S R Heard; B Rasburn; R C Matthews; S Tabaqchali
Journal:  J Clin Microbiol       Date:  1986-09       Impact factor: 5.948

5.  Molecular studies on the epidemiology and pathogenicity of Clostridium difficile.

Authors:  S Tabaqchali
Journal:  Gut       Date:  1989-11       Impact factor: 23.059

6.  Identification of toxigenic Clostridium difficile strains by using a toxin A gene-specific probe.

Authors:  B W Wren; C L Clayton; N B Castledine; S Tabaqchali
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

7.  Computer-aided densitometric analysis of protein patterns of Clostridium difficile.

Authors:  W Ehret; M Turba; P Pfaller; W Heizmann; G Ruckdeschel
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-04       Impact factor: 3.267

8.  Incidence and significance of Clostridium difficile in hospitalized cancer patients.

Authors:  M Gérard; N Defresne; D Daneau; P Van der Auwera; M Delmée; A M Bourguignon; F Meunier
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-04       Impact factor: 3.267

Review 9.  Automation in clinical microbiology: a new approach to identifying micro-organisms by automated pattern matching of proteins labelled with 35S-methionine.

Authors:  S Tabaqchali; R Silman; D Holland
Journal:  J Clin Pathol       Date:  1987-09       Impact factor: 3.411

10.  Differentiation of strains of Helicobacter pylori by numerical analysis of 1-D SDS-PAGE protein patterns: evidence for post-treatment recrudescence.

Authors:  M Costas; D D Morgan; R J Owen; D R Morgan
Journal:  Epidemiol Infect       Date:  1991-12       Impact factor: 2.451
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