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Literature DB >> 3700322

Reaggregation of fetal rat brain cells in a stationary culture system. II: Ultrastructural characterization.

Abstract

Ultrastructural characteristics of fetal rat brain cell aggregates in a three-dimensional stationary culture system are described. Transmission electron microscopy showed immature cells which developed into mature astrocytes, oligodendrocytes, and neurons during 20 d in culture. This was accompanied by the development of a neuropil where myelinated axons and synaptic complexes were observed. In addition to confirming earlier ultrastructural investigations on fetal rat brain cell aggregates, the stationary culture system also showed the presence of histiotypic regions within the aggregates. These regions consisted of ependymal cells where cilia were observed on the cell surfaces. Structures resembling subependymal basement membrane labyrinths were also observed. Macrophages seemed to be more numerous in the stationary cultures as compared to other culture systems. The stationary culture system may provide aggregates that are ultrastructurally more complex than those obtained by rotation mediated systems.

Entities: Disease Species

Mesh：

Year: 1986 PMID： 3700322 DOI： 10.1007/bf02623303

Source DB: PubMed Journal: In Vitro Cell Dev Biol ISSN： 0883-8364

15 in total

1. Morphological differentiation of mechanically dissociated fetal rat brain in aggregating cell cultures.

Authors: B D Trapp; P Honegger; E Richelson; H D Webster
Journal: Brain Res Date: 1979-01-05 Impact factor: 3.252

2. Sequence of developmental abnormalities leading to granule cell deficit in cerebellar cortex of weaver mutant mice.

Authors: P Rakic; R L Sidman
Journal: J Comp Neurol Date: 1973-11-15 Impact factor: 3.215

3. [Subependymal and interependymal labyrinths of basal membrane in ventricle system and central canal of the white rat].

Authors: K H Booz; U Desaga
Journal: Verh Anat Ges Date: 1972

4. Genotypes and phenotypes of ten inbred strains of BD-rats.

Authors: H Druckrey
Journal: Arzneimittelforschung Date: 1971-08

5. An electron microscopic study of the neuroglia during postnatal development of the rat cerebrum.

Authors: D W Caley; D S Maxwell
Journal: J Comp Neurol Date: 1968-05 Impact factor: 3.215

6. An electron microscopic study of neurons during postnatal development of the rat cerebral cortex.

Authors: D W Caley; D S Maxwell
Journal: J Comp Neurol Date: 1968-05 Impact factor: 3.215

7. Cell junction and ultrastructural development of reaggregated mouse brain cultures.

Authors: N W Seeds; S C Haffke
Journal: Dev Neurosci Date: 1978 Impact factor: 2.984

8. Recent observations on ependyma and subependymal basement membranes.

Authors: H Leonhardt; U Desaga
Journal: Acta Neurochir (Wien) Date: 1975 Impact factor: 2.216

9. Triodothyronine enhancement of neuronal differentiation in aggregating fetal rat brain cells cultured in a chemically defined medium.

Authors: P Honegger; D Lenoir
Journal: Brain Res Date: 1980-10-20 Impact factor: 3.252

10. Transformation of monocytes in tissue culture into macrophages, epithelioid cells, and multinucleated giant cells. An electron microscope study.

Authors: J S Sutton; L Weiss
Journal: J Cell Biol Date: 1966-02 Impact factor: 10.539

14 in total

Reaggregation of fetal rat brain cells in a stationary culture system. II: Ultrastructural characterization.

1. Morphological differentiation of mechanically dissociated fetal rat brain in aggregating cell cultures.

2. Sequence of developmental abnormalities leading to granule cell deficit in cerebellar cortex of weaver mutant mice.

3. [Subependymal and interependymal labyrinths of basal membrane in ventricle system and central canal of the white rat].

4. Genotypes and phenotypes of ten inbred strains of BD-rats.

5. An electron microscopic study of the neuroglia during postnatal development of the rat cerebrum.

6. An electron microscopic study of neurons during postnatal development of the rat cerebral cortex.

7. Cell junction and ultrastructural development of reaggregated mouse brain cultures.

8. Recent observations on ependyma and subependymal basement membranes.

9. Triodothyronine enhancement of neuronal differentiation in aggregating fetal rat brain cells cultured in a chemically defined medium.

10. Transformation of monocytes in tissue culture into macrophages, epithelioid cells, and multinucleated giant cells. An electron microscope study.

1. Adaptive adhesion systems mediate glioma cell invasion in complex environments.

2. Brain tumor invasion rate measured in vitro does not correlate with Ki-67 expression.

3. Heterogeneic modulation of malignant behavior in human glioma cells in defined and serum-containing media.

Review 4. Glial ontogeny and glial neoplasia: the search for closure.

5. Interaction between human brain tumour biopsies and fetal rat brain tissue in vitro.

6. Proliferation, migration, and invasion of human glioma cells exposed to fractionated radiotherapy in vitro.

7. Effects of growth factors on a human glioma cell line during invasion into rat brain aggregates in culture.

8. Migration, proliferation, and invasion of human glioma cells following treatment with simvastatin.

9. Interaction between human medulloblastomas and foetal rat brain aggregates in vitro.

10. Reaggregation of fetal rat brain cells in a stationary culture system. I: Methodology and cell identification.