Literature DB >> 3699043

DNA compaction during intense transcription measured by electron microscope tomography.

A L Olins, D E Olins, H A Levy, R C Durfee, S M Margle, E P Tinnel.   

Abstract

Thick sections (0.5-1.0 micron) containing pilocarpine-hyperstimulated Balbiani ring (BR) genes of Chironomus tentans salivary glands were reconstructed by electron microscope tomography (EMT). Six transcription loops were analyzed at low resolution permitting a measurement of transcription unit length in situ. Comparison of the average transcription unit length with the BR gene coding region DNA length yields an estimate of the DNA compaction ratio in situ. The estimated DNA compaction ratio (approximately 3-fold) is consistent with a significant degree of nucleosome unfolding in the hyperstimulated BR genes.

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Year:  1986        PMID： 3699043

Source DB:  PubMed          Journal:  Eur J Cell Biol        ISSN： 0171-9335            Impact factor:   4.492


  6 in total

1.  Electron spectroscopic imaging analyses of the distribution of phosphorus in Balbiani ring granules and in the surrounding nucleoplasm.

Authors:  G H Vázquez-Nin; S Abolhassani-Dadras; O M Echeverría; V B Rouelle-Rossier; M L von Schack; S Fakan
Journal:  Chromosoma       Date:  1997-04       Impact factor: 4.316

2.  The structure of nucleosomal core particles within transcribed and repressed gene regions.

Authors:  V M Studitsky; A V Belyavsky; A F Melnikova; A D Mirzabekov
Journal:  Nucleic Acids Res       Date:  1988-12-09       Impact factor: 16.971

3.  Electron microscope tomography of Balbiani Ring hnRNP substructure.

Authors:  A L Olins; D E Olins; H A Levy; M B Shah; D P Bazett-Jones
Journal:  Chromosoma       Date:  1993-01       Impact factor: 4.316

4.  Distribution of B52 within a chromosomal locus depends on the level of transcription.

Authors:  D T Champlin; J T Lis
Journal:  Mol Biol Cell       Date:  1994-01       Impact factor: 4.138

5.  Balbiani ring hnRNP substructure visualized by selective staining and electron spectroscopic imaging.

Authors:  A L Olins; D E Olins; D P Bazett-Jones
Journal:  J Cell Biol       Date:  1992-05       Impact factor: 10.539

6.  Large-scale chromatin structure of inducible genes: transcription on a condensed, linear template.

Authors:  Yan Hu; Igor Kireev; Matt Plutz; Nazanin Ashourian; Andrew S Belmont
Journal:  J Cell Biol       Date:  2009-04-06       Impact factor: 10.539
  6 in total

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