Literature DB >> 3693799

Rapid analysis of cellular lipids without extraction.

C Mazière1, J C Mazière, L Mora, J Polonovski.   

Abstract

A method is described where cell suspension obtained by scraping of monolayers was directly applied on silica gel plates. Extraction and separation of different lipid classes were simultaneously obtained during chromatography. In the range of validity of the method (no more than 80 micrograms of cellular protein tested for neutral lipids and 30 micrograms for phospholipids), this technique allows rapid lipid analysis of small samples of cultured cells, bypassing all the time- and solvent-consuming extraction and evaporation steps. The method appears to be also suitable for measurement of enzymes of lipid metabolism such as acyl coenzyme A-cholesterol-acyltransferase.

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Year:  1987        PMID： 3693799     DOI： 10.1016/0165-022x(87)90052-2

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN： 0165-022X


  8 in total

1.  The liver metabolite S-422 of the hypolipidaemic drug benfluorex decreases cholesterol esterification in fibroblasts and monocyte-like cells.

Authors:  J C Mazière; C Mazière; M Auclair; L Mora; O Arnaud
Journal:  Eur J Clin Pharmacol       Date:  1991       Impact factor: 2.953

2.  Inhibition of diphosphatidylglycerol synthesis by u.v. A radiations in N.C.T.C. 2544 human keratinocytes.

Authors:  M Djavaheri-Mergny; L Mora; C Mazière; M Auclair; R Santus; L Dubertret; J C Mazière
Journal:  Biochem J       Date:  1994-04-01       Impact factor: 3.857

3.  Palmitoylation of Semliki Forest virus glycoproteins in insect cells (C6/36) occurs in an early compartment and is coupled to the cleavage of the precursor p62.

Authors:  C G Schärer; H Y Naim; H Koblet
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

4.  Fatty-acid-binding proteins do not protect against induced cytotoxicity in a kidney cell model.

Authors:  A W Zimmerman; J H Veerkamp
Journal:  Biochem J       Date:  2001-11-15       Impact factor: 3.857

5.  Transfection of L6 myoblasts with adipocyte fatty acid-binding protein cDNA does not affect fatty acid uptake but disturbs lipid metabolism and fusion.

Authors:  C F Prinsen; J H Veerkamp
Journal:  Biochem J       Date:  1998-01-15       Impact factor: 3.857

6.  Modification of phospholipid polar head group with monomethylethanolamine and dimethylethanolamine decreases cholesteryl ester and triacylglycerol synthesis in cultured human fibroblasts.

Authors:  C Maziere; M Auclair; L Mora; J C Maziere
Journal:  Lipids       Date:  1990-06       Impact factor: 1.880

7.  Trifluoperazine increases fatty acid turnover in phospholipids in cultured human fibroblasts.

Authors:  C Mazière; J C Mazière; L Mora; M Auclair; J Polonovski
Journal:  Lipids       Date:  1988-05       Impact factor: 1.880

8.  The effects of perhexiline maleate on low density lipoprotein processing and cholesterol metabolism in cultured human fibroblasts.

Authors:  C Candide; J C Mazière; C Mazière; A Lageron; J Polonovski
Journal:  Eur J Clin Pharmacol       Date:  1988       Impact factor: 2.953
  8 in total

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