Fractionation of plant and animal high mobility group chromosomal proteins by ion-exchange and reversed-phase high-performance liquid chromatography.

A method for purifying wheat high mobility group (HMG) chromosomal proteins using a combination of weak cation-exchange and reversed-phase high-performance liquid chromatography (HPLC) is described. Previously reported HPLC systems devised for fractionating HMG proteins of vertebrate animals are not effective for the plant proteins. The system described here can be used for the fractionation of both plant and animal HMG proteins.