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Literature DB >> 3693402

Translocation of acyl-CoA oxidase into peroxisomes requires ATP hydrolysis but not a membrane potential.

Abstract

An efficient system for the import of newly synthesized proteins into highly purified rat liver peroxisomes was reconstituted in vitro. 35S-Labeled acyl-CoA oxidase (AOx) was incorporated into peroxisomes in a proteinase K-resistant fashion. This import was specific (did not occur with mitochondria) and was dependent on temperature, time, and peroxisome concentration. Under optimal conditions approximately 30% of [35S]AOx became proteinase resistant. The import of AOx into peroxisomes could be dissociated into two steps: (a) binding occurred at 0 degrees C in the absence of ATP; (b) translocation occurred only at 26 degrees C and required the hydrolysis of ATP. GTP would not substitute for ATP and translocation was not inhibited by carbonylcyanide-m-chlorophenylhydrazone, valinomycin, or other ionophores.

Entities: Chemical Gene Species

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Year: 1987 PMID： 3693402 PMCID： PMC2114735 DOI： 10.1083/jcb.105.6.2915

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

40 in total

1. Large cation-selective pores from rat liver peroxisomal membranes incorporated to planar lipid bilayers.

Authors: P Labarca; D Wolff; U Soto; C Necochea; F Leighton
Journal: J Membr Biol Date: 1986 Impact factor: 1.843

2. Permeability of the peroxisomal membrane to cofactors of beta-oxidation. Evidence for the presence of a pore-forming protein.

Authors: P P Van Veldhoven; W W Just; G P Mannaerts
Journal: J Biol Chem Date: 1987-03-25 Impact factor: 5.157

3. Protein import into chloroplasts requires a chloroplast ATPase.

Authors: D Pain; G Blobel
Journal: Proc Natl Acad Sci U S A Date: 1987-05 Impact factor: 11.205

4. Proton ionophores prevent assembly of a peroxisomal protein.

Authors: E Bellion; J M Goodman
Journal: Cell Date: 1987-01-16 Impact factor: 41.582

5. Transport of F1-ATPase subunit beta into mitochondria depends on both a membrane potential and nucleoside triphosphates.

Authors: N Pfanner; W Neupert
Journal: FEBS Lett Date: 1986-12-15 Impact factor: 4.124

Review 6. Biogenesis of peroxisomes.

Authors: P B Lazarow; Y Fujiki
Journal: Annu Rev Cell Biol Date: 1985

7. ATP is essential for protein translocation into Escherichia coli membrane vesicles.

Authors: L Chen; P C Tai
Journal: Proc Natl Acad Sci U S A Date: 1985-07 Impact factor: 11.205

8. Synthesis of 3-ketoacyl-CoA thiolase of rat liver peroxisomes on free polyribosomes as a larger precursor. Induction of thiolase mRNA activity by clofibrate.

Authors: Y Fujiki; R A Rachubinski; R M Mortensen; P B Lazarow
Journal: Biochem J Date: 1985-03-15 Impact factor: 3.857

9. The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions.

Authors: F Leighton; B Poole; H Beaufay; P Baudhuin; J W Coffey; S Fowler; C De Duve
Journal: J Cell Biol Date: 1968-05 Impact factor: 10.539

10. Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

Authors: Y Fujiki; A L Hubbard; S Fowler; P B Lazarow
Journal: J Cell Biol Date: 1982-04 Impact factor: 10.539

52 in total

1. Pex15p of Saccharomyces cerevisiae provides a molecular basis for recruitment of the AAA peroxin Pex6p to peroxisomal membranes.

Authors: Ingvild Birschmann; An K Stroobants; Marlene van den Berg; Antje Schäfer; Katja Rosenkranz; Wolf-H Kunau; Henk F Tabak
Journal: Mol Biol Cell Date: 2003-03-07 Impact factor: 4.138

Translocation of acyl-CoA oxidase into peroxisomes requires ATP hydrolysis but not a membrane potential.

1. Large cation-selective pores from rat liver peroxisomal membranes incorporated to planar lipid bilayers.

2. Permeability of the peroxisomal membrane to cofactors of beta-oxidation. Evidence for the presence of a pore-forming protein.

3. Protein import into chloroplasts requires a chloroplast ATPase.

4. Proton ionophores prevent assembly of a peroxisomal protein.

5. Transport of F1-ATPase subunit beta into mitochondria depends on both a membrane potential and nucleoside triphosphates.

Review 6. Biogenesis of peroxisomes.

7. ATP is essential for protein translocation into Escherichia coli membrane vesicles.

8. Synthesis of 3-ketoacyl-CoA thiolase of rat liver peroxisomes on free polyribosomes as a larger precursor. Induction of thiolase mRNA activity by clofibrate.

9. The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions.

10. Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

1. Pex15p of Saccharomyces cerevisiae provides a molecular basis for recruitment of the AAA peroxin Pex6p to peroxisomal membranes.

Review 2. Peroxisome biogenesis in Saccharomyces cerevisiae.

Review 3. Import of proteins into peroxisomes and other microbodies.

Review 4. On the translocation of proteins across the chloroplast envelope.

Review 5. Targeting of proteins into the peroxisomal matrix.

6. Mapping the cargo protein membrane translocation step into the PEX5 cycling pathway.

Review 7. The surprising complexity of peroxisome biogenesis.

8. Import of a DHFR hybrid protein into glycosomes in vivo is not inhibited by the folate-analogue aminopterin.

Review 9. In vitro systems in the study of peroxisomal protein import.

10. Catalase-negative peroxisomes in human embryonic liver.