Literature DB >> 3691814

Rapid characterization of mucin oligosaccharides from rat small intestine with gas chromatography-mass spectrometry.

H Karlsson1, I Carlstedt, G C Hansson.   

Abstract

Mucin glycopeptides were isolated from rat small intestinal mucosa after reduction/alkylation, trypsin digestion and gel chromatography. The oligosaccharides were released by using alkaline-NaBH4, separated into neutral and acidic species and permethylated. The derivatized mixtures were analysed with fast atom bombardment mass spectrometry and gas chromatography-mass spectrometry using thin film columns. Permethylated neutral oligosaccharides with up to seven sugars could be chromatographed and detected with mass spectrometry. The complex mixture revealed was partly due to the linkage GalNAc being substituted at both position 3 and 6. The approach will be very useful when analysing small amounts of mucins and mucin fragments.

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Year:  1987        PMID： 3691814     DOI： 10.1016/0014-5793(87)80543-4

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  4 in total

1.  High-temperature gas chromatography and gas chromatography-mass spectrometry of glycoprotein and glycosphingolipid oligosaccharides.

Authors:  H Karlsson; N Karlsson; G C Hansson
Journal:  Mol Biotechnol       Date:  1994-04       Impact factor: 2.695

2.  Strategy for the investigation of O-linked oligosaccharides from mucins based on the separation into neutral, sialic acid- and sulfate-containing species.

Authors:  N G Karlsson; H Karlsson; G C Hansson
Journal:  Glycoconj J       Date:  1995-02       Impact factor: 2.916

3.  Two glycosylation alterations of mouse intestinal mucins due to infection caused by the parasite Nippostrongylus brasiliensis.

Authors:  Jessica M Holmén; Fredrik J Olson; Hasse Karlsson; Gunnar C Hansson
Journal:  Glycoconj J       Date:  2002-01       Impact factor: 2.916

4.  Electron ionization-tandem mass spectrometry of glycosphingolipids. I: The identiftcation of compound-specific sequence ions in the collision-induced dissociation spectra of the immonium ions of two isomeric hexaglycosylceramides.

Authors:  J M Curtis; P J Derrick; J Holgersson; B E Samuelsson; M E Breimer
Journal:  J Am Soc Mass Spectrom       Date:  1992-05       Impact factor: 3.109
  4 in total

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