Literature DB >> 3689349

A simple procedure for the isolation of protein disulphide-isomerase.

J Koivu1, R Myllylä, K I Kivirikko.   

Abstract

A two-step procedure is described for the purification of protein disulphide-isomerase (PDI). This procedure is based on the previous finding that the beta-subunit of the prolyl 4-hydroxylase tetramer (alpha 2 beta 2) is identical with PDI [Koivu, Myllylä, Helaakoski, Pihlajaniemi, Tasanen & Kivirikko (1987) J. Biol. Chem. 262, 6447-6449; Pihlajaniemi, Helaakoski, Tasanen, Myllylä, Huhtala, Koivu & Kivirikko (1987) EMBO J. 6, 643-649]. The procedure involves purification of the prolyl 4-hydroxylase tetramer by a simple affinity chromatography and subsequent isolation of the beta-subunit from the dissociated tetramer by ion-exchange chromatography.

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Year:  1987        PMID： 3689349      PMCID： PMC1148394          DOI： 10.1042/bj2470237

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  21 in total

1.  Thiol-protein disulphide oxidoreductases. Assay of microsomal membrane-bound glutathione-insulin transhydrogenase and comparison with protein disulphide-isomerase.

Authors:  A L Ibbetson; R B Freedman
Journal:  Biochem J       Date:  1976-11       Impact factor: 3.857

2.  Purification and partial characterization of the two nonidentical subunits of prolyl hydroxylase.

Authors:  R A Berg; N L Kedersha; N A Guzman
Journal:  J Biol Chem       Date:  1979-04-25       Impact factor: 5.157

Review 3.  Experimental and theoretical aspects of protein folding.

Authors:  C B Anfinsen; H A Scheraga
Journal:  Adv Protein Chem       Date:  1975

4.  Purification and characterization of a thiol:protein disulfide oxidoreductase from bovine liver.

Authors:  D F Carmichael; J E Morin; J E Dixon
Journal:  J Biol Chem       Date:  1977-10-25       Impact factor: 5.157

5.  Posttranslational enzymes in the biosynthesis of collagen: intracellular enzymes.

Authors:  K I Kivirikko; R Myllylä
Journal:  Methods Enzymol       Date:  1982       Impact factor: 1.600

6.  An improved method for the purification of vertebrate prolyl hydroxylase by affinity chromatography.

Authors:  N L Kedersha; R A Berg
Journal:  Coll Relat Res       Date:  1981-07

7.  Immunological evidence for an inactive precursor of collagen proline hydroxylase in cultured fibroblasts.

Authors:  J O McGee; U Langness; S Udenfriend
Journal:  Proc Natl Acad Sci U S A       Date:  1971-07       Impact factor: 11.205

8.  Ascorbate increases the synthesis of procollagen hydroxyproline by cultured fibroblasts from chick embryo tendons without activation of prolyl hydroxyla.

Authors:  W W Kao; R A Berg; D J Prockop
Journal:  Biochim Biophys Acta       Date:  1975-12-05

9.  An affinity-column procedure using poly(L-proline) for the purification of prolyl hydroxylase. Purification of the enzyme from chick embryos.

Authors:  L Tuderman; E R Kuutti; K I Kivirikko
Journal:  Eur J Biochem       Date:  1975-03-03

10.  Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene.

Authors:  T Pihlajaniemi; T Helaakoski; K Tasanen; R Myllylä; M L Huhtala; J Koivu; K I Kivirikko
Journal:  EMBO J       Date:  1987-03       Impact factor: 11.598
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