Literature DB >> 3688427

Assay of tryptophan decarboxylase from Catharanthus roseus plant cell cultures by high-performance liquid chromatography.

E J Pennings1, I Hegger, R van der Heijden, J A Duine, R Verpoorte.   

Abstract

An assay is described for the enzyme tryptophan decarboxylase from plant cell suspension cultures. It is based on the fluorometric detection of tryptamine by HPLC on a LiChrosorb RP-8 Select B column. Tryptophan decarboxylase from Catharanthus roseus was induced by transferring 14-day-old cells into an induction medium. Optimum activity was found 2 days after transfer, the increase being 5- to 10-fold. When kept at -15 degrees C the crude enzyme lost half its activity in about 7 days. The rate of the decarboxylation reaction was linear for at least 3 h at 35 degrees C.

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Year:  1987        PMID： 3688427     DOI： 10.1016/0003-2697(87)90210-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine glucosidase in suspension cultured cells of Catharanthus roseus and Tabernaemontana divaricata.

Authors:  L H Stevens; T J Blom; R Verpoorte
Journal:  Plant Cell Rep       Date:  1993-08       Impact factor: 4.570

2.  Expression of two consecutive genes of a secondary metabolic pathway in transgenic tobacco: molecular diversity influences levels of expression and product accumulation.

Authors:  M J Leech; K May; D Hallard; R Verpoorte; V De Luca; P Christou
Journal:  Plant Mol Biol       Date:  1998-11       Impact factor: 4.076

3.  Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density.

Authors:  P R Moreno; J E Schlatmann; R van der Heijden; W M van Gulik; H J ten Hoopen; R Verpoorte; J J Heijnen
Journal:  Appl Microbiol Biotechnol       Date:  1993-04       Impact factor: 4.813

4.  A chimaeric tryptophan decarboxylase gene as a novel selectable marker in plant cells.

Authors:  O J Goddijn; P M van der Duyn Schouten; R A Schilperoort; J H Hoge
Journal:  Plant Mol Biol       Date:  1993-08       Impact factor: 4.076

5.  Influence of Precursor Availability on Alkaloid Accumulation by Transgenic Cell Line of Catharanthus roseus

Authors: 
Journal:  Plant Physiol       Date:  1998-02-01       Impact factor: 8.340

6.  Tryptophan decarboxylase plays an important role in ajmalicine biosynthesis in Rauvolfia verticillata.

Authors:  Wanhong Liu; Rong Chen; Min Chen; Haoxing Zhang; Meifang Peng; Chunxian Yang; Xingjia Ming; Xiaozhong Lan; Zhihua Liao
Journal:  Planta       Date:  2012-02-14       Impact factor: 4.116

7.  Elicitor-mediated induction of isochorismate synthase and accumulation of 2,3-dihydroxy benzoic acid in Catharanthus roseus cell suspension and shoot cultures.

Authors:  P R Moreno; R van der Heijden; R Verpoorte
Journal:  Plant Cell Rep       Date:  1994-12       Impact factor: 4.570

8.  Overexpression of a tryptophan decarboxylase cDNA in Catharanthus roseus crown gall calluses results in increased tryptamine levels but not in increased terpenoid indole alkaloid production.

Authors:  O J Goddijn; E J Pennings; P van der Helm; R A Schilperoort; R Verpoorte; J H Hoge
Journal:  Transgenic Res       Date:  1995-09       Impact factor: 2.788

9.  Targeting tryptophan decarboxylase to selected subcellular compartments of tobacco plants affects enzyme stability and in vivo function and leads to a lesion-mimic phenotype.

Authors:  Stefano Di Fiore; Qiurong Li; Mark James Leech; Flora Schuster; Neil Emans; Rainer Fischer; Stefan Schillberg
Journal:  Plant Physiol       Date:  2002-07       Impact factor: 8.340

10.  The role of aromatic L-amino acid decarboxylase in bacillamide C biosynthesis by Bacillus atrophaeus C89.

Authors:  Lei Yuwen; Feng-Li Zhang; Qi-Hua Chen; Shuang-Jun Lin; Yi-Lei Zhao; Zhi-Yong Li
Journal:  Sci Rep       Date:  2013       Impact factor: 4.379
  10 in total

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