A peroxide-induced inflammation model for drug testing.

Peroxide-induced cellular injury is an important mediator of inflammation. Whether the injection of glucose oxidase (GO) with its generation of H2O2 may be utilized as an inflammatory model was studied. Anesthesized mice were injected in the right hind foot with either water or an equal volume of 10 or 100 units/ml GO. The animals were sacrificed at 1.5 or 24 hours post injection and both hind feet amputated and each foot weighed. Difference in weights for each animal's feet was called edema index (EI). GO produced significant increases in weight. The use of GO to produce inflammation may be useful in testing potential peroxide quenching agents.